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The terms 5' and 3' in DNA structure refer to the carbon atoms in the sugar backbone of the DNA molecule. The 5' end has a phosphate group attached to the 5th carbon atom, while the 3' end has a hydroxyl group attached to the 3rd carbon atom. This directional orientation is important because DNA strands are antiparallel, meaning they run in opposite directions. This arrangement allows for the complementary base pairing between the strands, which is essential for DNA replication and protein synthesis.

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