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DNA polymerase III requires a primer, which is a short piece of RNA or DNA, in order to function correctly.

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4mo ago

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What is the function of a DNA?

There are different types of DNA polymerase depending if it's from a eukaryotic or prokaryotic cell each performing specific tasks. Basically DNA polymerase catalyzes the formation of a polymer, a DNA strand, from many monomers, deoxyribonucleotides.


Role of magnesium chloride in PCR?

Magnesium chloride is a crucial component in the polymerase chain reaction (PCR) as it is required for the activity of the DNA polymerase enzyme. Magnesium ions help stabilize the DNA template-primer complex and are essential for the enzymatic activity of the DNA polymerase, allowing for successful DNA amplification during PCR. The optimal concentration of magnesium chloride can vary depending on the specific DNA polymerase being used and the PCR conditions.


Where does RNA polymerase bind?

RNA polymerase bind specific regions of DNA called promoters. The RNA polymerase holoenzyme is guided to promoters by interactions between members of the holoenyzme and specific DNA sequences such as the TATA box.


What are the different types of RNA polymerases and their promoters?

There are three different types of RNA polymerases in eukaryotic cells: RNA polymerase I, II, and III. Each type is responsible for transcribing a specific set of genes. RNA polymerase I transcribes ribosomal RNA genes, RNA polymerase II transcribes protein-coding genes, and RNA polymerase III transcribes small structural RNA genes. The promoters for each type of RNA polymerase are different and contain specific sequences that are recognized by the polymerase to initiate transcription.


What is the source of the polymerase used in polymerase chain reaction?

The polymerase used in polymerase chain reaction (PCR) is typically derived from a thermophilic bacterium called Thermus aquaticus. The specific polymerase most commonly used is Taq polymerase, which is known for its ability to withstand high temperatures required for PCR.


Where does RNA polymerase bind to the DNA to initiate transcription?

RNA polymerase binds to the DNA at a specific region called the promoter to initiate transcription.


Where does RNA polymerase start the transcription process?

RNA polymerase starts the transcription process at a specific DNA sequence called the promoter region.


What is primer in pcr?

A primer (oligonucleotide of a specific sequence) is required for Taq polymerase to extend the template strand by adding complementary nucleotides. The function of the primer is to anneal to the template strand at a very specific site and facilitate the initiation of strand elongation mediated by Taqploymerase.


Which organelle is correctly paired with is specific function?

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What is a polarised component?

A polarized component is an electronic device that can only be connected in a specific orientation to function properly. This ensures that current flow and voltage are in the correct direction. Examples of polarized components include electrolytic capacitors, diodes, and LEDs.


What genetic locus serves as a recognition site for RNA polymerase?

The promoter region, typically located upstream of the coding sequence, serves as the recognition site for RNA polymerase. It contains specific DNA sequences that allow RNA polymerase to bind and initiate transcription.


What is The nontranscribed region of DNA to which RNA polymerase binds to initiate transcription called?

The nontranscribed region of DNA to which RNA polymerase binds to initiate transcription is called the promoter region. It contains specific sequences that signal to RNA polymerase where to begin transcribing the gene.