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What do we get from Transcription?

Processed mRNA (methylated cap, poly-A tail, introns removed, exons rearranged)

What else do we need?

Ribosomes

tRNA

Amino acids

Amino acyl tRNA synthase

Peptidyl transferase

What are we trying to make?

Proteins

Here are the steps for translation, rather briefly:

1) Amino acyl tRNA synthase matches amino acids to their respective tRNA molecules.

2) Activated tRNA-amino acid complexes are moved to the ribosome (either in the cytoplasm or on the Rough Endoplasmic Reticulum). Processed mRNA also moves to the ribosome.

3) Ribosome fixes onto the mRNA strand and detects the start codon AUG.

4) When it hits AUG, the tRNA coding for Methionine will enter the A site of the ribosome. Here, the tRNA carries out complementary base pairing with the mRNA strand.

5) The Met-tRNA moves to the P site. The next codon (3 bps) are read and the appropriate tRNA molecule binds to it in the A site.

6) The Met amino acid from the P site then binds to the 2nd amino acid from the A site, catalysed by peptidyl transferase. The first tRNA molecule leaves the ribosome through the E site. The second tRNA molecule moves into the P site.

7) The step above is repeated until the ribosome gets to the STOP codon. When this occurs, the ribosome leaves the mRNA strand and the mRNA strand disintegrates. The completed polypeptide chain folds into its secondary, tertiary and quarternary structures and forms proteins and enzymes.

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Q: What are in biology the steps of translation?
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