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How to test for protein - The Biuret test

1. Add Sodium hydroxide to a 1% solution of albumen (the protein in egg white) or the ground food you want to be tested for protein

2. Then add 1% copper sulphate solution

3. If protein is present the solution will go purple

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Related Questions

What is the use of the tests for proteins?

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If Biuret and Millon's test give positive result what is the conclusion?

If both Biuret and Millon's tests give a positive result, it suggests the presence of proteins in the sample. Biuret test is specifically for proteins containing peptide bonds, while Millon's test is used for identifying proteins with tyrosine residues. The positive results from both tests provide strong evidence for the presence of proteins in the sample.


Which protein show millions test?

Proteins with the capability to bind to specific molecules or structures such as antibodies, enzymes, or transcription factors can be used in various types of tests, including diagnostic tests or research assays. Proteins like immunoglobulins, enzymes like polymerases or ligases, and transcription factors like RNA polymerase are commonly used in millions of tests worldwide due to their specificity and utility in detecting or measuring a wide range of molecules.


Do proteins give positive iodine tests?

Yes, proteins can give a positive iodine test. When iodine reacts with proteins, it forms a complex that results in a brown color change, indicating a positive test for proteins.


Does Benedict's solution reacts with proteins?

Benedict's solution reacts with reducing sugars, not proteins. It is commonly used in biochemical tests to detect the presence of reducing sugars such as glucose. Proteins do not typically react with Benedict's solution.


What is the basis for the xanthoproteic and biuret tests?

The xanthoproteic test is based on the reaction between phenolic amino acids, such as tyrosine, and concentrated nitric acid, which produces yellow-colored nitrophenolic compounds, indicating the presence of proteins. The biuret test, on the other hand, relies on the formation of a violet complex when proteins containing peptide bonds react with copper sulfate in an alkaline solution. Both tests are used to detect and confirm the presence of proteins in a sample.


When a quantitative tests are needed for different substances what urine specimen is usually requested?

random,fasting timed, 24 hour, catheterized


Biochemical tests are used to determine what?

Biochemical tests are used to determine the presence of specific molecules, such as proteins, enzymes, or metabolites, in a sample. These tests help to diagnose diseases, monitor treatment, and assess overall health status based on the chemical reactions happening in the body.


What laboratory tests require blood collected in a heparin tube?

Laboratory tests that require blood collected in a heparin tube include activated partial thromboplastin time (aPTT), thrombin time, and some specialized tests for specific proteins involved in blood clotting. Heparin is an anticoagulant that prevents blood from clotting and is often used in tests that require plasma samples.


What do activators do?

They help the blood sample in the tube clot so that serum can be separated for analysis. Serum is the preferred specimen for many tests such as hormones, proteins and electrolytes.


How can food tests be used to distinguish between unknown food samples?

Food tests can be used to identify specific macromolecules present in unknown food samples by applying biochemical assays that indicate the presence of carbohydrates, proteins, and lipids. For example, the Benedict's test can detect reducing sugars, while the iodine test is used for starch, and the Biuret test identifies proteins. Additionally, the grease spot test can indicate the presence of fats. By combining these tests, one can create a profile of the unknown sample, aiding in its identification.


What is visceral proteins?

Visceral proteins are a group of proteins produced by the liver that are involved in various physiological processes, including immune response and transport of molecules in the blood. Their levels can be measured in blood tests to assess nutritional status and liver function. Examples include albumin, prealbumin, and transferrin.