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Advantages: Nuclear chromatin detail are well presented together with cytoplasmic counterstaining and cytoplasmic transparancy is obtained.

Disadvantages: Need for rapid fixation to preserve nuclear features, cells can float off the slide, in prominent morulae formations like adenocarcenoma the dark nuclear staining obscures detailed nuclear morphology, thicker areas of the smear often artefactually take up orange stain.

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Q: What are the Advantages and disadvantaged of a pap stain?
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