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1)Allow transfer of genes from bacterium to plant by the use of plasmid, known as Ti plasmid.
2) Allow to be used as a tool for plant breeding e.g. any desired gene, such as insecticidal toxin genes or herbicide resistant genes, can be engineered into the bacterial DNA then transferred into plant genome
3) Agrobacterium shortens the conventional plant breeding process
4) Allows entirely new (non-plant) genes to be engineered into crops
5) Is a highly effective biological control system for disease (e.g. crown gall disease).
6)Used for plant improvement (2 above)
What else can I help you with?
Compare the transformation of a bacterium cell with the transformation of a plant cell?
In bacteria, if the plasmid containing the foreign DNA manages to get inside a bacterial cell, this sequence ensures that it will be replicated. In Plant Cells, if transformation is successful the recombinant DNA is integrated into one of the chromosomes of the cell.
Is Agrobacterium tumifacians a fungi?
No, Agrobacterium tumefaciens is a bacterium, not a fungus. It is a soil-dwelling bacterium that is known for its ability to transfer DNA between itself and plants, causing the formation of plant tumors.
What is the process of inserting this gene into dna of a tomato plant is known as?
The process of inserting a gene into the DNA of a tomato plant is known as genetic engineering or genetic modification. This is typically done using techniques such as biolistics (gene gun), Agrobacterium-mediated transformation, or CRISPR-Cas9 gene editing.
How can new genes be introduced into a plant to enhance its characteristics or traits?
New genes can be introduced into a plant through a process called genetic engineering. This involves inserting the desired gene into the plant's DNA using techniques like gene guns or Agrobacterium tumefaciens. The introduced gene can enhance the plant's characteristics or traits, such as improving its resistance to pests or increasing its yield.
What is the function of the Ti plasmid?
The Ti plasmid is a circular DNA molecule found in Agrobacterium species. It serves as a vector for transferring genes into plant cells, leading to the formation of crown gall tumors. The transferred genes help the bacterium infect and genetically modify the plant cells to its advantage.
Why is liquid nitrogen used in agrobacterium transformation?
Liquid nitrogen is used in agrobacterium transformation to create small, transient pores in plant cells through a process called cryoporation. These pores allow the agrobacterium to deliver DNA to the plant cell more efficiently, increasing the success rate of genetic transformation. Liquid nitrogen also helps in maintaining a low temperature environment, which is necessary for cell survival during the transformation process.
Does transformation by Agrobacterium work best in monocotyledon plants?
no
What method is preferred to generate transgenic plants?
Since transgenic plants contains foreign gene of desired function of other organisms.So the technique of rDna technology is used.Then the rDNA is cultured in the lab then after the formation of small plant it is grown in the outside entertainment.in this way a transgenic plant is produced.
How can genetic engineering be used to make a transgenic tobacco plant that glows in the dark?
Agrobacterium-mediated transformation using a plasmid containing a gene for GFP
Agrobacterium?
produce plant tumors to gain nutrient -> These tumors are often fatal to plant
The Ti plasmid from which plant pathogen is used to transfer plant genes between species?
The Ti plasmid is derived from Agrobacterium tumefaciens, which is a plant pathogen. This plasmid is commonly used as a vector to transfer foreign genes into plant cells in genetic engineering applications.
Which method is preferred to generate transgenic plants?
Agrobacterium-mediated transformation is often preferred for generating transgenic plants because it allows for the stable integration of DNA into the plant genome with a high efficiency. This method has been widely adopted due to its ability to transfer DNA into a wide range of plant species with minimal impact on the plant's genome.
What is an agroinfiltration?
An agroinfiltration is the introduction of a suspension of agrobacterium into a plant to add a specific gene.
What is recombinant Ti plasmid used for?
Recombinant Ti plasmids are used in genetic engineering to introduce foreign DNA into plant cells. This is done by Agrobacterium-mediated transformation, where the Ti plasmid delivers the desired DNA into the plant genome, allowing for the expression of novel traits or genes in the plant.
What hormone directly activated vir gene in agrobacterium Ti plasmid?
The hormone that directly activates the vir gene in Agrobacterium Ti plasmid is acetosyringone. It induces the expression of the vir genes, triggering the transfer of T-DNA from Agrobacterium to plant cells.
Why gene gun use in rice transformation?
It's a tool to insert foregin gene into target cells and can be used for any suitable crop or cells for transformation purpose. Now a days, agrobacterium mediated transformation techniques are becoming popular.
Compare the transformation of a bacterium cell with the transformation of a plant cell?
In bacteria, if the plasmid containing the foreign DNA manages to get inside a bacterial cell, this sequence ensures that it will be replicated. In Plant Cells, if transformation is successful the recombinant DNA is integrated into one of the chromosomes of the cell.
