What are the steps of manipulating a microscope?

Answer 1

A system and method are disclosed which enable manipulation of a sample under study with a microscope. In one embodiment, a manipulation system is adaptable for interfacing with any of a plurality of different types of microscopes, such as a transmission electron microscope (TEM) and a scanning electron microscope (SEM), and further comprises at least one manipulation mechanism operable to manipulate a sample. In another embodiment, a manipulation system is capable of being detachably coupled to a microscope, such as a TEM, and comprises a plurality of manipulator mechanisms for manipulating a sample. In a preferred embodiment, the manipulation system comprises both an adjustable interface such that it is capable of selectively coupling with any of a plurality of different microscope interfaces and a plurality of manipulator mechanisms integrated therein that are controllably operable for manipulating a sample. == * Fatikow, et al., "A Flexible Microrobot-Based Microassembly Station," Emerging Technologies and Factory Automation, 1999. Proceedings ETFA '99. 1999 7th IEEE International Conference, Barcelona, Spain 18-21 Oct. 1999, Piscataway, NJ USA, pp. 397-406. * Investment Approaches: Lux NanoSphere: Measurement, Nanotechnology, The Nanotech Report 2001, pp. 122-136. * Technical Data from Klocke Nanotechnik, Dr. Volker Klocke, Aachen, Germany, printed Nov. 2002, 85 pages. * U.S. appl. No. 10/173,543, Min-Feng Yu. * Yu, Minfeng, et al., "Three-dimensional manipulation of carbon nanotubes under a scanning electron microscope", 1999 IOP Publishing Ltd., pp. 244-25

Answer 2

Manipulate a Microscope

Instructions

1. • 1

     Set up the microscope on a flat surface. Always carry the microscope using two hands if the microscope must be transported from one area to another. One hand should support the bottom while the other hand is holding the arm. Once in place, remove the any covers and plug in the microscope.

   • 2

     Position the slide onto the stage. Clip it into or under the slide holders. Turn on the light from the illuminator under the stage. Center the slide the best way possible without using any focusing knobs or the eyepiece.

   • 3

     Look through the eyepiece, keeping both eyes open to avoid eye strain, while using the course focus knob to bring the stage and slide closer to the objective, which is the lens that extends below the eyepiece, until the object can be seen through the eyepiece. Use the fine focus knob (which will also be on the side of the microscope) to bring the image into sharper focus.

   • 4

     Center the object being viewed. Change the iris diaphragm under the stage using the level to open and close the iris. Change the amount of light entering the specimen as needed. Changing the amount of light going through the slide may show additional details not seen at higher light levels.

   • 5

     Change to the 10X objective which is the next longest lens by rotating the lens pieces. The object should appear larger and only require using the fine focus knob to bring it into sharper detail. Use the iris diaphragm to change the amount of light entering the slide. Continue to the 40X objective and repeat. Each time the object will appear larger and you will be able to see less of the object at any one time while viewing.

   • 6

     Lower the stage and add one or two drops of immersion oil to the slide. Change the microscope to the 100X objective. Slowly raise the stage using the course focus until the objective is touching the oil. Use the fine focus knob to bring the object into sharp focus. Clean the slide and objective with lens tissue after use.

   • 7

     Return the stage to the lowest level. Change the objective back to the 4X lens. Unplug the machine and replace any covers. Carefully return the microscope using both hands to where it is stored.

Answer 3

1. Place the slide on the stage of the microscope.
2. Adjust the focus using the coarse adjustment knob for initial focusing and the fine adjustment knob for finer details.
3. Adjust the light source and the diaphragm to optimize the illumination.
4. Use the magnification objectives to increase the magnification of the specimen.

Answer 4

how do you manipulate the microscope?

Answer 5

You move the knobs!