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What is the difference between Puc18 and Puc19?
The multiple cloning sites (MCS) in pUC18 and pUC19 is the difference - the MCS in pUC19 is reverse orientated to those of the pUC18.pUC18: LacZ HindIII PaeI ..... SacI EcoRIpUC19: Lacz EcoRI SacI ..... PaeI HindIII
Explain the principle of insertional inactivation by giving a suitable example?
insertional inactivation is basically about the inactive of second marker where the protein cannot be longer produced. the suitable example is pUC19 where the lacZ gene that encoded for beta-galactosidase cannot be longer produced when the foreign gene is inserted in it..
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