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A smear in your PCR can be caused by too low annealing temperature. Too much or little magnesium or poor primer design or most likely poorly extracted DNA for your template. Precipitate your template DNA again with 70% ethanol give it a good dry and re-elute and try once more. You can also try diluting your template. If there are PCR inhibitors in your DNA prep then using less template can improve results. If you have plenty of DNA in your prep then a 10 or even 100 fold dilution shouldn't prevent good amplification.
What else can I help you with?
How do the A band and I band in skeletal muscle contribute to the overall structure and function of a muscle band?
The A band and I band in skeletal muscle play important roles in the structure and function of a muscle band. The A band contains thick myosin filaments and overlaps with thin actin filaments from the I band during muscle contraction. The I band contains only thin actin filaments and helps maintain the structure of the muscle band. Together, the A band and I band work to generate force and movement in the muscle during contraction.
The area of the sarcomere containing the thick filaments is the?
The area of the sarcomere containing the thick filaments is known as the A band. It appears dark under a microscope due to the overlapping thick filaments. The A band also includes some thin filaments at its edges where they overlap with the thick filaments.
What are the differences between the a and i bands in terms of their function and structure?
The A band is the dark band in a sarcomere that contains thick myosin filaments and does not change length during muscle contraction. The I band is the light band that contains thin actin filaments and shortens during muscle contraction. The A band provides stability and structure, while the I band allows for muscle contraction and relaxation.
What can you assume is contained within each band?
Band 1: Introductory information and background about the topic. Band 2: Main arguments, supporting points, and examples. Band 3: Further insights, analysis, and implications. Band 4: Conclusion or summary of key points and possible next steps.
What are the most effective exercises for targeting the L band muscle?
The most effective exercises for targeting the L band muscle are lateral band raises, side plank variations, and clamshells. These exercises specifically engage and strengthen the L band muscle, which is important for hip stability and overall lower body strength.
When did Clear - Christian band - end?
Clear - Christian band - ended in 2001.
When was Clear - Christian band - created?
Clear - Christian band - was created in 1997.
How can you change the wrist band for your?
the question is not clear
What does the clear silly band mean?
I is just a color.
What species of the Plasmodium species has band forms?
Plasmodium falciparum is the species known to have band forms. These are elongated forms of asexual parasites seen in the peripheral blood smear of infected individuals with malaria.
Allergic to adhesive band aids what can I use instead?
Every pharmacy has Allergenic band aids.
If the boiler smoke is not clear you should?
adjust the air band
Which band has got a music video where the band is playing there instruments in a clear box?
The only band I can think of is Battles and the music video song is Atlas.
What causes ringoworm?
kicking Pete best out of the band
What is used in a marching band instead of a tuba?
Sousaphones, Or Contras
Are rock band drums compatible with rock band 2?
Yes they are but instead of asking why dont you just try it out? =]
Why does Jupiter have a band appearance?
The difference in atmosphere density and what's in the air causes it to form into band like formation
