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A smear in your PCR can be caused by too low annealing temperature. Too much or little magnesium or poor primer design or most likely poorly extracted DNA for your template. Precipitate your template DNA again with 70% ethanol give it a good dry and re-elute and try once more. You can also try diluting your template. If there are PCR inhibitors in your DNA prep then using less template can improve results. If you have plenty of DNA in your prep then a 10 or even 100 fold dilution shouldn't prevent good amplification.
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The area of the sarcomere containing the thick filaments is the?
I believe that would be in the sarcomeres of muscle cells.
What causes the space or gap between front teeth defined as frenum a genetic thing or from disease processes?
The frenum or frenulum linguae is the band of tissue beneath the tongue. The frenulum labii superioris is the band of tissue behind your upper lip. Neither have anything to do with the gap between your central incisors, which is called diastema or gap-tooth. The gap is probably genetic in most cases.
Is allele a Christian band?
no they are mainstream
How do Tendons in the hip and pelvic area affect pain.?
my hip is bone on bone, I was born with it, as I get older it is more painful.. Need hip surgery but have no insurance. Thinking of changing pain medication. What causes the pain in your groin that feels like a tight rubber band. and why to I have pain on the outside of my thigh and down past my knee?
Who are the beatels?
The beatles are a famos rock band
When did Clear - Christian band - end?
Clear - Christian band - ended in 2001.
When was Clear - Christian band - created?
Clear - Christian band - was created in 1997.
How can you change the wrist band for your?
the question is not clear
What does the clear silly band mean?
I is just a color.
If the boiler smoke is not clear you should?
adjust the air band
Allergic to adhesive band aids what can I use instead?
Every pharmacy has Allergenic band aids.
Which band has got a music video where the band is playing there instruments in a clear box?
The only band I can think of is Battles and the music video song is Atlas.
What causes ringoworm?
kicking Pete best out of the band
What is used in a marching band instead of a tuba?
Sousaphones, Or Contras
Are rock band drums compatible with rock band 2?
Yes they are but instead of asking why dont you just try it out? =]
Why does Jupiter have a band appearance?
The difference in atmosphere density and what's in the air causes it to form into band like formation
What are the causes of it band pain?
One of the most known causes of it band pain would be because of the inflammation of the iliotibial band. Mostly the people that have this are runners and athletes. People who go to the gym should change up their exercise because if not, then this could occur.
