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A smear in your PCR can be caused by too low annealing temperature. Too much or little magnesium or poor primer design or most likely poorly extracted DNA for your template. Precipitate your template DNA again with 70% ethanol give it a good dry and re-elute and try once more. You can also try diluting your template. If there are PCR inhibitors in your DNA prep then using less template can improve results. If you have plenty of DNA in your prep then a 10 or even 100 fold dilution shouldn't prevent good amplification.

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How do the A band and I band in skeletal muscle contribute to the overall structure and function of a muscle band?

The A band and I band in skeletal muscle play important roles in the structure and function of a muscle band. The A band contains thick myosin filaments and overlaps with thin actin filaments from the I band during muscle contraction. The I band contains only thin actin filaments and helps maintain the structure of the muscle band. Together, the A band and I band work to generate force and movement in the muscle during contraction.


The area of the sarcomere containing the thick filaments is the?

The area of the sarcomere containing the thick filaments is known as the A band. It appears dark under a microscope due to the overlapping thick filaments. The A band also includes some thin filaments at its edges where they overlap with the thick filaments.


What are the differences between the a and i bands in terms of their function and structure?

The A band is the dark band in a sarcomere that contains thick myosin filaments and does not change length during muscle contraction. The I band is the light band that contains thin actin filaments and shortens during muscle contraction. The A band provides stability and structure, while the I band allows for muscle contraction and relaxation.


What can you assume is contained within each band?

Band 1: Introductory information and background about the topic. Band 2: Main arguments, supporting points, and examples. Band 3: Further insights, analysis, and implications. Band 4: Conclusion or summary of key points and possible next steps.


What are the most effective exercises for targeting the L band muscle?

The most effective exercises for targeting the L band muscle are lateral band raises, side plank variations, and clamshells. These exercises specifically engage and strengthen the L band muscle, which is important for hip stability and overall lower body strength.