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If the Gram Stain is completed properly, gram positive should stain purple; however, if you over decolorize a gram positive organism, the organism will appear appear pink, which is a gram negative reaction. To summarize, if you over decolorize a gram positive organism it will show as a gram negative organism.

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13y ago
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9y ago

During a gram stain, gram negative organisms would normally show up pink. If you under-decolorize them, it would turn purple but if you over-decolorized the gram negative stain it will take on a red color from safranin.

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14y ago

gram posotive are thick wall bacterial cells which can retain the colour of methylene blue even after decolourization and so the did not take the counter stain on them
so after counterstaining they appear either blue or purple in colour

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12y ago

if we over decolorize the gram negative bacteria it appears colorless but it appears pink only when it stained with saffranine

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14y ago

Pinkish/Red

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Q: What color are gram-negative organisms if you over-decolorized them?
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