What distinguish a protein isolation from the isolation of small oraganic molecule?

Answer 1

The isolation (or extraction) of proteins requires a different method that those for organic molecules.

Proteins are extremely sensitive to temperature and ionic conditions. Therefore, the protocol for the isolation of a protein should take into consideration the preparation of the correct buffers and experimental methods to keep the protein intact so it does not degrade during the extraction process.

Protein are typically extracted from live cells so the isolation protocol should include disrupting the cell and removing the protein undamaged.

With organic molecules however, liquid-liquid extraction or 2-phase extraction may be carried out (depending on the properties of the organic substance)

Answer 2

Protein isolation typically involves techniques such as chromatography and centrifugation to separate proteins based on their size or charge, whereas the isolation of small organic molecules often involves solvent extraction or distillation techniques. Proteins are larger and more complex molecules compared to small organic molecules, which requires different methods for isolation. Additionally, proteins may require careful handling to maintain their structure and function during isolation.