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What does Transcription in eukaryotes require in addition to RNA polymerase?
Wiki User
∙ 12y ago
General Transcription Factors (GTF). TFIID (binds to DNA first at TATA box), TFIIA, TFIIB, TFIIF, TFIIE and TFIIH.
Wiki User
∙ 12y ago
Wiki User
∙ 13y agoA helicase is required to unwind the DNA, a primase to create a primer, and a polymerase to lengthen the mRNA.
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Why eukaryotes need 3 RNA polymerase?
Each of the 3 RNA polymerase in eukaryotes perform a different function. For example, RNA pol 2 is involved in mRNA synthesis, RNA pol 1 synthesizes most rRNAs and RNA pol 3 synthesizes tRNNA, the 5S rRNA and other small nuclear RNAS. Eukaryotes are more complex than prokaryotes, hence why eukaryotes would require more RNA polymerases.
Transcription?
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Differences between DNA polymerase and RNA polymerase?
A polymerase is an enzyme that catalyzes the conversion of free nucleotides into a single strand. DNA polymerase differs from RNA polymerase in two major respects: * Like all enzymes, DNA polymerase is substrate-specific. DNA polymerase cannot extend a single strand of DNA; it needs at least a short segment of double-stranded DNA at the outset. * As its name implies, DNA polymerase incorporates deoxyribonucleotides into the new strand. RNA polymerase incorporates ribonucleotides. These differences mean that DNA polymerase is active when new DNA strands are formed, as in DNA replication, and RNA polymerase is active when new RNA is formed, as in transcription. Before DNA replication can begin, the two strands must uncoil, so that each can form a template for free nucleotides to attach to. But DNA polymerase cannot get started with a single strand! In vivo(in the cell) RNA polymerase, which is active in the presence of single-stranded DNA, catalyzes the incorporation of a handful of nucleotides into a new strand. The short length of double-stranded nucleic acid that is produced enables DNA polymerase to swing into action. This still leaves a potential difficulty: the nucleotides incorporated in the presence of RNA polymerase are the wrong sort (ribonucleotides). They are subsequently replaced by DNA polymerase. In vitro (during PCR, the polymerase chain reaction) a primer, specially synthesized in a laboratory, attaches to a specific segment of single-stranded DNA, and the DNA polymerase takes over from there. The primer consists of a short length of single-stranded DNA that uniquely complements a specific DNA segment that is targeted for amplification, for example for forensic analysis.In practice, there are several different DNA polymerases and RNA polymerases in an organism.
What enzyme connects the new bases to the old bases in the DNA template?
The central enzyme involved is DNA polymerase, which catalyzes the joining of deoxyribonucleoside 5′-triphosphates (dNTPs) to form the growing DNA chain.
Why do you need to add primers DNA polymerase and nucleotides to your PCR mixture?
NEED OF PRIMER IN PCR-It is because the polymerase enzyme we use in the PCR only extend a DNA strand but not initiate its synthesis. So, to initiate the synthesis of DNA strand onto a template strand we require primers.
Does initiation of transcription require a primer?
no because RNA polymerase can do the same thing
Why eukaryotes need 3 RNA polymerase?
Each of the 3 RNA polymerase in eukaryotes perform a different function. For example, RNA pol 2 is involved in mRNA synthesis, RNA pol 1 synthesizes most rRNAs and RNA pol 3 synthesizes tRNNA, the 5S rRNA and other small nuclear RNAS. Eukaryotes are more complex than prokaryotes, hence why eukaryotes would require more RNA polymerases.
Transcription?
Get StartedThis letter can used to request that your high school or college transcript be sent to another high school, college or university, or other organization, such as a prospective employer.To assist in the search of your records, you should include the time period that you attended the institution.In some instances, there may be a fee involved in sending the transcript.
What happens to the chromatin?
The structure of chromatin during interphase is optimized to allow easy access of transcription and DNA repair factors to the DNA while compacting the DNA into the nucleus. The structure varies depending on the access required to the DNA. Genes that require regular access by RNA polymerase require the looser structure provided by euchromatin
Does transcription require DNA?
No, Transcription does not require Replication to take place first. Transcription is simply the process of making mRNA from DNA so that the ribosomes have directions to make proteins. Replication is the complete copy of the genetic material in the host chromosome which would occur before the cell divides.
Which processes do NOT require DNA replication?
Dna transcription, the production of messenger Rna.
What is accomplished by transcription?
By the end of transcription, mRNA will be available for the next step which is translation. You require mRNA to translate into a protein for a specific function the cell needs to carry out.
Differences between DNA polymerase and RNA polymerase?
A polymerase is an enzyme that catalyzes the conversion of free nucleotides into a single strand. DNA polymerase differs from RNA polymerase in two major respects: * Like all enzymes, DNA polymerase is substrate-specific. DNA polymerase cannot extend a single strand of DNA; it needs at least a short segment of double-stranded DNA at the outset. * As its name implies, DNA polymerase incorporates deoxyribonucleotides into the new strand. RNA polymerase incorporates ribonucleotides. These differences mean that DNA polymerase is active when new DNA strands are formed, as in DNA replication, and RNA polymerase is active when new RNA is formed, as in transcription. Before DNA replication can begin, the two strands must uncoil, so that each can form a template for free nucleotides to attach to. But DNA polymerase cannot get started with a single strand! In vivo(in the cell) RNA polymerase, which is active in the presence of single-stranded DNA, catalyzes the incorporation of a handful of nucleotides into a new strand. The short length of double-stranded nucleic acid that is produced enables DNA polymerase to swing into action. This still leaves a potential difficulty: the nucleotides incorporated in the presence of RNA polymerase are the wrong sort (ribonucleotides). They are subsequently replaced by DNA polymerase. In vitro (during PCR, the polymerase chain reaction) a primer, specially synthesized in a laboratory, attaches to a specific segment of single-stranded DNA, and the DNA polymerase takes over from there. The primer consists of a short length of single-stranded DNA that uniquely complements a specific DNA segment that is targeted for amplification, for example for forensic analysis.In practice, there are several different DNA polymerases and RNA polymerases in an organism.
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What enzyme connects the new bases to the old bases in the DNA template?
The central enzyme involved is DNA polymerase, which catalyzes the joining of deoxyribonucleoside 5′-triphosphates (dNTPs) to form the growing DNA chain.
What is RNA polymerase and what is its job?
The RNA polymerases are huge multi-subunit protein complexes. Three kinds are found in eukaryotes: * RNA polymerase I (Pol I).It transcribes the rRNA genes for the precursor of the 28S, 18S, and 5.8S molecules (and is the busiest of the RNA polymerases). * RNA polymerase II (Pol II; also known as RNAP II).It transcribes protein-encoding genes into mRNA (and also the snRNA genes). * RNA polymerase III (Pol III).It transcribes the 5S rRNA genes and all the tRNAgenes.
