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Which job must enzymes do before replication can begin?
They must unwind part of the original DNA molecule.
What molecule is first active during DNA replication?
helicase enzymes
How do restriction enzymes specifically target and cut DNA molecules?
Restriction enzymes recognize specific sequences of nucleotides in DNA molecules, called recognition sites. These enzymes then bind to these sites and cut the DNA at specific points within or near the recognition site, resulting in the cleavage of the DNA molecule.
What do the bands on a restriction map show?
The bands on a restriction map show the sizes of DNA fragments after they have been cut by restriction enzymes. These bands represent the different DNA fragments that result from the digestion of a DNA molecule with specific restriction enzymes at their recognition sites. The pattern of bands can be used to determine the order and distances between restriction sites on the DNA molecule.
How do restriction enzymes precisely cut DNA molecules?
Restriction enzymes recognize specific sequences of nucleotides in DNA molecules and bind to them. Once bound, the enzyme cuts the DNA at specific points within or near the recognized sequence, resulting in precise cleavage of the DNA molecule.
What molecule controls production of enzymes?
DNA
Nucleic acids are synthesized directly on the DNA molecule with the help of what?
enzymes
What is the role in enzymes in the DNA replication process?
Enzymes split the DNA molecule into two rails and then transport corresponding nitrogen bases to each rail.
What breaks the bonds between the nitrogen bases of the DNA molecule?
Your answer is "Helicase". This is the enzyme responsible for the unzipping of the DNA molecule, or in other words, the breakage of the bonds of its nitrogen bases.
Which job must enzymes do before replication can begin?
They must unwind part of the original DNA molecule.
What molecule is first active during DNA replication?
helicase enzymes
How do restriction enzymes specifically target and cut DNA molecules?
Restriction enzymes recognize specific sequences of nucleotides in DNA molecules, called recognition sites. These enzymes then bind to these sites and cut the DNA at specific points within or near the recognition site, resulting in the cleavage of the DNA molecule.
What is responsible for building the DNA strand?
DNA is build from another template DNA molecule using DNA polymerase, among other enzymes.
What do the bands on a restriction map show?
The bands on a restriction map show the sizes of DNA fragments after they have been cut by restriction enzymes. These bands represent the different DNA fragments that result from the digestion of a DNA molecule with specific restriction enzymes at their recognition sites. The pattern of bands can be used to determine the order and distances between restriction sites on the DNA molecule.
Of what biological significance is the DNA molecule?
The biological significance of a DNA molecule is that it: 1) Controls the synthesis of proteins (as well as enzymes because they are also proteins). 2) Is responsible for carrying hereditary characteristics.
Why restriction enzyme is also called endonuclease enzyme?
Because these enzymes cut the DNA molecule at a particular site. But like scissors these are useful tools in genetic engineering or recombinant DNA technology.
What is the difference between Restriction endonucleases and exonucleases?
Exonuclease enzymes cleave nucleotides from the ends of DNA molecules. Endonuclease enzymes cleave a phosphodiester bond somewhere within the DNA molecule (not at the ends).
