function of eosin methylene blue agar (EMB)
Emb agar
The conclusion drawn if no growth appeared on MacConkey agar and EMB agar after inoculation of the media and an incubation period could be the bacteria used was possibly a Gram positive non-enteric sample.
So here is the deal, same use different recipies different results to do the same thing. I'll just post some lecture notes and hope that helps:MacConkey's Agar (enterics)Selective for gram negativeCrystal violet and bile salts prevent non-entericsDifferential for lactose fermentationNon-fermenter --> white/clear coloniesFermenter--> red/pink coloniesEosin Methylene Blue (EMB) (Weakly selective for intestinal pathogens)Selective for gram negativeEosin and methylene blue inhibit gram positive growthDifferential for lactose fermentationLactose fermenters produce a green-metallic sheenNon-fermenters do notNOTE: EMB Agar is not in common use anymore
Escherichia coli-Salmonella-Shigella-Enterobacter
No, it will not. Methylene blue agar is used to identify gram negative bacteria, staphylococcus is gram positive.
yes it can be grow in EMB agar.
Emb agar
Colorless, it doesnt ferment lactose.
Gram + bacteria do NOT grow on EMB agar, EMB agar inhibits their growth, and is selective for Gram -
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EMB agar or Eosin methylene blue agar is to see which gram negative is lactose fermenters by colonies appearing a purple, black color. It also inhibits the growth of gram positive bacteria.
EMB Agar
The conclusion drawn if no growth appeared on MacConkey agar and EMB agar after inoculation of the media and an incubation period could be the bacteria used was possibly a Gram positive non-enteric sample.
The conclusion drawn if no growth appeared on MacConkey agar and EMB agar after inoculation of the media and an incubation period could be the bacteria used was possibly a Gram positive non-enteric sample.
methylene blue
function of agar slopes
So here is the deal, same use different recipies different results to do the same thing. I'll just post some lecture notes and hope that helps:MacConkey's Agar (enterics)Selective for gram negativeCrystal violet and bile salts prevent non-entericsDifferential for lactose fermentationNon-fermenter --> white/clear coloniesFermenter--> red/pink coloniesEosin Methylene Blue (EMB) (Weakly selective for intestinal pathogens)Selective for gram negativeEosin and methylene blue inhibit gram positive growthDifferential for lactose fermentationLactose fermenters produce a green-metallic sheenNon-fermenters do notNOTE: EMB Agar is not in common use anymore