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Molecular carriers such as NADH donate electrons and Hydrogen ions through the Electron Transport Chain which is located across the inner membrane of the mitochondrion and consists of Complexes I, II, III & IV.

The H ions are pumped out of the inner membrane against their natural concentration gradient, whilst the electrons are transported through the various complexes. The donated electrons are eventually accepted by Oxygen molecules in Complex IV.

The H ions that have previously been pumped out of the inner membrane build up to form a pH and electrochemical gradient so that the inside of the inner membrane is alkaline and negatively charged. This is known as the Proton Motive Force.

The H ions travel back into the inner membrane through another complex called the ATP Synthase molecule (or ATPase). As these ions travel through the ATPase they provide energy which allows Adenosine diphosphate (ADP) to bind with inorganic phosphate (Pi) to produce Adenosine triphosphate (ATP).

Simply put, the Electron Transport Chain creates a Proton Motive Force which allows the creation of ATP from ADP and Pi.

Read up on the bold terms to get a more indepth understanding of ATP production.

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15y ago

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