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Robert Koch (1843-1910) was a German physician and microbiologist who showed that "the key to the identification of bacterial pathogens was the isolation of pure cultures."

What are Koch's postulates?

In the course of his studies of anthrax and tuberculosis, Robert Koch formulated rules of procedure for proving that a certain microorganism is the cause of a particular disease. These rules, known as Koch's postulates, are still used today:

1. It must be shown that the microorganism in question is always present in diseased hosts.

2. The microorganism must be isolated from the diseased host and grown in pure culture (i.e., in a culture containing only that one species of microorganism).

3. Microorganisms obtained from the pure culture, when injected into a healthy, susceptible host, must produce the disease in that host.

4. Microorganisms must be isolated from the experimentally infected host, grown in pure culture, and compared with the microorganisms from the original culture.

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