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Krebs-Henseleit buffer was developed in the early

1930's by Hans Krebs and Kurt Henseleit. This

modification of Ringer's solution was used to maintain

liver tissue during experiments that led Krebs to

postulate the urea cycle. The formula offered by

Sigma has been modified by the addition of 2 g/L of

glucose as an energy source for cell maintenance, and

by the omission of calcium chloride.

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12y ago

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