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What is the difference between Wright stain and Giemsa stain?
The Wright stain is a combination of eosin and methylene blue dyes, while Giemsa stain is a mixture of eosin, methylene blue, and azure dyes. Giemsa stain is commonly used for staining blood smears to visualize parasites and bacteria, while Wright stain is used more for general cell morphology in blood and bone marrow specimens.
What is the principle of giemsa stain?
Giemsa stain is a type of Romanowsky stain used in cytogenetics and histopathology to stain chromosomes, blood cells, and microorganisms. It works by binding to the phosphate groups in nucleic acids, such as DNA and RNA, allowing for visualization of cellular structures under a microscope.
How is the Giemsa stain used in the diagnosis of malaria?
The Giemsa stain is used in the diagnosis of malaria by staining the parasites in a blood sample, making them visible under a microscope. This helps healthcare providers identify the presence of malaria parasites in the blood, aiding in the accurate diagnosis of the disease.
What is the difference between leishman staining and giemsa staining?
Leishman staining is used for staining blood in microscopy and its purpose is to both identify and differentiate trypanosomas, leucocytes and malaria parasites. Giesma staining is used to stain DNA region, specifically chromosomes in order to locate aberrations like rearrangement and translocations.
What is the advantages and disadvantages of Giemsa stain?
* Cells relatively large * Cytoplasm well displayed even in lymphocyte, oat cells,…etc. * Good for showing basophilia, cytoplasmic granules, and surface microvilia * Signs of degeneration easily visible * If mounted without delay, dyes fast for several decades. * Heterochromatin not shown in form familiar to histopathologist. * Hyperchromasia not recognizable * Nucleoli pale * Free cells may appear adherent * Three-dimensional structures not transparent * Slides useless if cells not concentrated enough to dry rapidly
What is the difference between Wright stain and Giemsa stain?
The Wright stain is a combination of eosin and methylene blue dyes, while Giemsa stain is a mixture of eosin, methylene blue, and azure dyes. Giemsa stain is commonly used for staining blood smears to visualize parasites and bacteria, while Wright stain is used more for general cell morphology in blood and bone marrow specimens.
What region of the DNA does the giemsa stain dye?
Giemsa stain binds the regions with high content of adenine-thymine complex.
What is the principle of giemsa stain?
Giemsa stain is a type of Romanowsky stain used in cytogenetics and histopathology to stain chromosomes, blood cells, and microorganisms. It works by binding to the phosphate groups in nucleic acids, such as DNA and RNA, allowing for visualization of cellular structures under a microscope.
What stain do you use to see banding patterns in chromosomes?
Giemsa is the most common stain used to see banding patterns in chromosomes
How is the Giemsa stain used in the diagnosis of malaria?
The Giemsa stain is used in the diagnosis of malaria by staining the parasites in a blood sample, making them visible under a microscope. This helps healthcare providers identify the presence of malaria parasites in the blood, aiding in the accurate diagnosis of the disease.
Why use methanol to fix smear during giemsa staining?
because giemsa stain is a mixture of methyl acetate Eosin and azure b. it doesnot contain any fixative that is why we use methanol to fix smear during giemsa stain other stain like lieshman contain acetyl free methyl alcohol as a fixative so it does not need to fix slide stain with lieshman stain.
Wrights stain or other stains?
Wright's stain is a Romanowsky stain used in medical laboratories to differentiate blood cells for microscopy. Other stains used for blood cell differentiation include Giemsa stain, Leishman stain, and Hematoxylin and eosin stain. Each stain has specific applications and staining properties.
When was Gustav Giemsa born?
Gustav Giemsa was born in 1867.
When did Gustav Giemsa die?
Gustav Giemsa died in 1948.
When did Edmund Giemsa die?
Edmund Giemsa died in 1994.
When was Edmund Giemsa born?
Edmund Giemsa was born in 1912.
What is the difference between leishman staining and giemsa staining?
Leishman staining is used for staining blood in microscopy and its purpose is to both identify and differentiate trypanosomas, leucocytes and malaria parasites. Giesma staining is used to stain DNA region, specifically chromosomes in order to locate aberrations like rearrangement and translocations.
