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Think of the repressor as an on off switch for radio

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What prevents e coli from expressing the lac genes in its DNA all of the time?

The lac genes in E. coli are regulated by the lac operon, which is controlled by a repressor protein. The repressor binds to the operator region of the DNA, blocking the transcription of the lac genes. When lactose is present, it binds to the repressor, causing a conformational change that releases the repressor from the operator, allowing for the expression of the lac genes.


What genes is not a part of the lac operon?

repressor gene


A lac repressor turns ff the lac genes by binding to?

A lac repressor turns off the lac genes by binding to the operator


What does the lac operon lactose bind to?

The lac operon is a group of genes involved in metabolizing lactose. The protein Lac repressor binds to the operator site in the absence of lactose, blocking gene expression. When lactose is present, it binds to the Lac repressor, causing it to release from the operator and allowing transcription of the genes involved in lactose metabolism.


Describe the effect a repressor has on the lac operon when lactose is present?

When lactose is present, it binds to the repressor protein, causing a conformational change that prevents the repressor from binding to the operator region of the lac operon. As a result, RNA polymerase can transcribe the structural genes of the lac operon, leading to the production of enzymes involved in lactose metabolism.


What causes the lac genes in E. coli to turn off?

A lac repressor turns off the lac genes by binding to the operator.


What is a clue that the lac operon is on?

One clue that the lac operon is on is the presence of lactose in the environment. The lac operon is induced when lactose is available as a substrate for the lac repressor protein, allowing transcription of genes involved in lactose metabolism.


How does lactose cause the lac operon to turn on?

The lac repressor protein has a binding site for lactose itself.


What is the function of the operator locus of a Lac operon a. Binding with RNA polymerase b. Identifying the substrate lactose c. Producing messenger RNA d. Coding for represser e. Binding wit?

a. Incorrect, the promoter binds RNAP b. Incorrect, the lac repressor identifies lactose c. Incorrect, the RNAP produces mRNA from the lacY, lacZ and lacA genes d. Incorrect, lacI codes for the repressor e. ?? By the process of elimination this would be the correct answer but the operator locus binds the *lac repressor* ("wit" is an unfamiliar designation).


Transcription of lactose-metabolizing genes is blocked when?

Transcription of lactose-metabolizing genes is blocked when the Lac repressor protein binds to the operator region of the lac operon. This typically occurs in the presence of glucose when lactose is absent or in low concentrations. The Lac repressor prevents RNA polymerase from transcribing the genes, leading to inhibition of lactose metabolism.


What is the difference between the induction mechanisms of IPTG and lactose in gene expression regulation?

IPTG is a synthetic inducer that directly binds to the lac repressor protein, preventing it from blocking the lac operon. Lactose, on the other hand, is a natural inducer that is converted into allolactose, which then binds to the lac repressor to release it from the lac operon.


How does the presence of lactose enable RNA polymerase to transcribe the lac genes?

The presence of lactose enables RNA polymerase to transcribe the lac genes by inducing a conformational change in the lac repressor protein. This change prevents the lac repressor from binding to the lac operator, allowing RNA polymerase to access the promoter region and initiate transcription of the lac genes.