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Technical replicates: measure quantity from 1 source. This measures the reproducibility of the results. The differences are based only on technical issues in the measurement. (I weigh myself three times, do I get different weights? How different?)

Biological replicates: measure a quantity from difference sources under the same conditions. Tumors from 5 different people with lung cancer may show similar gene expression patterns. These replicates are useful to show what is similar in your replicates and how they are different from a different set of conditions (ie. treated, normal).

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