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Bacteria with the F plasmid (free or integrated) make pili (singular pilus, sometimes referred to as the sex pilus) to connect with bacteria that do not have the F plasmid to initiate DNA transfer. However, the pili are actually used as a harpoon to reel in the other bacteria so that physical contact between the membranes of the bacteria could be made. DNA is only transferred after the fusion of the cell membranes. It is also a common misconception to think that bacterial conjugation is like 'sex' since it does not involve recombination of genes or cycles of haploidy and diploidy.

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What occurs during conjugation?

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