What is the advantages and disadvantages of Giemsa stain?
* Cells relatively large
* Cytoplasm well displayed even in lymphocyte, oat
cells,…etc.
* Good for showing basophilia, cytoplasmic granules, and surface
microvilia
* Signs of degeneration easily visible
* If mounted without delay, dyes fast for several decades.
* Heterochromatin not shown in form familiar to
histopathologist.
* Hyperchromasia not recognizable
* Nucleoli pale
* Free cells may appear adherent
* Three-dimensional structures not transparent
* Slides useless if cells not concentrated enough to dry
rapidly