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It depends on the species and culture conditions, but single LPS molecules generally range from 4kDa to 20kDa. However, they readily form micelles that can be from 300kDa to 1000kDa.

Petsch, D. and Anspach, F.B., Endotoxin removal from protein solutions. J. Biotechnol. 76, 97-119 (2000).

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Q: What is molecular weight of LPS in Bacteria species?
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Why are gram positive bacteria more heat resistant than gram negative bacteria?

Gram positive bacteria contain more peptidoglycan than gram negative bacteria which is the substance responsible for the increased strength of gram positive bacterias cell walls therefore making them more resistant to heat. Gram negative do contain peptidoglycan but much, much less


What is an outer membrane?

The bacterial outer membrane is found in Gram-negative bacteria. Its composition is distinct from that of the cytoplasmic membrane - among other things, the outer leaflet of the outer membrane of many Gram-negative bacteria includes a complex lipopolysaccharide whose lipid portion acts as an endotoxin - and in some bacteria such as E. coli it is linked to the cell's peptidoglycan by Braun's lipoprotein.Porins can be found in this layer.[1]Contents[show] 1 Clinical significance2 Biogenesis3 See also4 ReferencesClinical significanceIf lipid A, part of the LPS, enters the circulatory system it causes a toxic reaction by activating TLR 4. Lipid A is very immunogenic and causes an aggressive response by the immune system. The sufferer will have a high temperature and respiration rate and a low blood pressure. This may lead to endotoxic shock, which may be fatal. BiogenesisThe biogenesis of the outer membrane requires that the individual components are transported from the site of synthesis to their final destination outside the inner membrane by crossing both hydrophilic and hydrophobic compartments. The machinery and the energy source that drive this process are not yet fully understood. The lipid A-core moiety and the O-antigen repeat units are synthesized at the cytoplasmic face of the inner membrane and are separately exported via two independent transport systems, namely, the O-antigen transporter Wzx (RfbX) and the ATP binding cassette (ABC) transporter MsbA that flips the lipid A-core moiety from the inner leaflet to the outer leaflet of the inner membrane.[2][3][4][5][6] O-antigen repeat units are then polymerised in the periplasm by the Wzy polymerase and ligated to the lipid A-core moiety by the WaaL ligase.[7][8] The LPS transport machinery is composed of LptA, LptB, LptC, LptD, LptE. This supported by the fact, that depletion of any of one of these proteins blocks the LPS assembly pathway and results in very similar outer membrane biogenesis defects. Moreover, the location of at least one of these five proteins in every cellular compartment suggests a model for how the LPS assembly pathway is organised and ordered in space.[8]LptC is equired for the translocation of lipopolysaccharide (LPS) from the inner membrane to the outer membrane.[8] LptE forms a complex with LptD, which is involved in the assembly of LPS in the outer leaflet of the outer membrane and is essential for envelope biogenesis.[8][9][10]


Which structure of bacteria is determied by gram staining?

The part of the bacterial cell that appears to play the most important role in determining whether an organism if gram-positive or not is the cell wall. it is determined by whether or not it retains the stain during testing.


Are bacteria similar to and different from protozoans?

Bacteria are prokaryotes, meaning they have no membrane-bound nuclei, or organelles. They are also single celled, and typically autotrophic, which means that is can make its own food. Protozoa are eukaryotic organisms, meaning they have a membrane-bound nucleus. It is also single celled, but it is a heterotroph, meaning it cannot make its own food.


What is the differences between gram positive and gram negative cells?

Gram was a scientist who invented a technique called Gram staining by which bacteria can be colorized and divided into two groups.Gram positiveappearance: blue to transfer to purpleStructure: Have thick layer of peptidoglycan over inner cytoplasmic membrane.They lack LPS-lipopolysaccharides.Gram negativeappearance: pink to transfer to redStructure: In gram negative bacteria the peptidoglycan layer is thinner and is located between space of the outer and inner cytoplasmic membrane.The cell wall contains LPS, which make them virulent.

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What is the function of LPS or Lipopolysaccharide?

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What ic lps?

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koala elephant platypus dog jaguar rooster baboon