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SST is an integral procedure to be done in every drug product analysis (qualitative or quantitative). USP-(621) Chromatography gives the requirements for SST and acceptance criteria, unless it is specified in the specific USP-Monograph. Getting the results conform SST requirements means that you get the permission to go ahead with analysis the contrary means that some changes must be done within the frame of USP-(621) Chromatography. Normally with SST parameters we understand what is required by USP, EP,BP,JP,DAB, that is: %RSD<2%(5 injections) and %RSD>2%(6 injections), retention factor>2,tailing factor<1.5, selectivity>1 and resolution>1.5. Having such parameters means that system as whole works properly and you can proceed with the analysis.
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Why uracil is used in hplc calibration?
Uracil is used as a standard reference compound in high-performance liquid chromatography (HPLC) calibration because it has well-defined retention characteristics and a simple chromatographic profile. Uracil is often used to determine retention times and assess the performance of the HPLC system.
What is AUFS in hplc?
AUFS stands for Agilent Universal Fit Sample loop. It is a type of sample loop used in high-performance liquid chromatography (HPLC) systems. AUFS sample loops are designed to be compatible with various types of HPLC systems, providing flexibility and ease of use.
What are the key differences between FPLC and HPLC, and how do these differences impact their respective applications in chromatography?
FPLC (fast protein liquid chromatography) and HPLC (high-performance liquid chromatography) are both chromatography techniques used to separate and analyze compounds. The key differences lie in their intended applications and the types of samples they are best suited for. FPLC is primarily used for purifying proteins and other biomolecules, while HPLC is more versatile and can be used for a wider range of compounds including small molecules, peptides, and proteins. FPLC typically operates at lower pressures and flow rates compared to HPLC, making it gentler on biomolecules. These differences impact their applications in chromatography by determining which type of sample each technique is best suited for. FPLC is ideal for purifying proteins and biomolecules, while HPLC is better suited for a broader range of compounds. The choice between FPLC and HPLC depends on the specific requirements of the experiment and the nature of the sample being analyzed.
How can HPLC be utilized for protein purification?
High Performance Liquid Chromatography (HPLC) can be used for protein purification by separating proteins based on their chemical properties, such as size, charge, and hydrophobicity. This technique allows for the isolation of specific proteins from a complex mixture, making it a powerful tool in biochemistry and biotechnology.
How can I use the steady state calculator to determine the equilibrium point of a system?
To determine the equilibrium point of a system using a steady state calculator, input the system's equations and parameters into the calculator. The calculator will then solve for the values of the variables at which the system reaches equilibrium, known as the equilibrium point. This point represents the stable state of the system where there is no change over time.
What is system suitability?
System suitability is a set of established criteria that ensure an analytical method is performing correctly and providing reliable results before sample analysis begins. It involves testing the performance of the system (such as HPLC or GC) to ensure it meets predetermined acceptance criteria regarding resolution, peak symmetry, and sensitivity. System suitability tests are essential for ensuring the reliability and accuracy of analytical data.
What are the HPLC Calibration parameters and elaborate it?
1. Flow rate 2. Temp. of column 3. Detector function 4. Resolution
Where can one purchase a Hitachi HPLC system?
There are a few places where one can purchase a Hitachi HPLC system. Some websites which sells these include Hitachi-hta, Absclex, eBay, Jascoinc, and Labcompare.
How do you optimise peak separation in HPLC?
Peak separation in HPLC can be optimized by adjusting parameters such as column length, particle size, mobile phase composition, flow rate, and temperature. Increasing column length can improve separation, while decreasing particle size can enhance resolution. Adjusting mobile phase composition can also impact peak separation by changing selectivity. Optimization is typically achieved through systematic experimentation and adjustment of these parameters.
Why propyl paraben used in HPLC calibration?
Propylparaben is used as a preservative in solutions for High Performance Liquid Chromatography (HPLC) calibration to prevent microbial growth and maintain stability of the calibration standards over time. Its use helps ensure the accuracy and reliability of the HPLC analysis results by preventing degradation of the calibration standards.
What is post run in HPLC?
Post run in HPLC refers to the time after the completion of a chromatographic analysis where the system continues running to ensure that any remaining compounds are fully flushed out of the column and detector to prevent contamination and achieve a clean baseline for subsequent runs. It is an important step to maintain the integrity and performance of the HPLC system.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
What are the validation parameters for related substance by hplc analysis?
The validation parameters for related substances analysis by HPLC typically include specificity, linearity, accuracy, precision, detection limit, quantification limit, and robustness. Specificity ensures the method can differentiate between the analyte and impurities, while linearity confirms a linear relationship between concentration and response. Accuracy and precision assess the closeness of results to the true value and the method's repeatability. Detection and quantification limits determine the lowest concentration that can be reliably detected and quantified. Robustness evaluates the method's ability to remain unaffected by small variations in parameters.
How do you determine lauric acid in coconut milk using hplc?
To determine lauric acid in coconut milk using HPLC, you would first extract the fatty acids from the coconut milk using a suitable solvent. Then, prepare the HPLC system with a column and mobile phase that can separate lauric acid from other compounds present. Inject the extracted sample into the HPLC system and use a suitable detector to quantify the amount of lauric acid present based on retention time and peak area.
What would operational testers use to assess whether a system meets usability reliability and maintainability parameters?
Measures of Suitability (MOSs) are specific, objective measures of how well as system can be maintained and utilized by the end user. They are written and approved by an independent operational test agency. An example of a MOS is: "Aircraft Mean Time Between Failure (MTBF)."
How do you calculate concentration from peak area in HPLC analysis?
To calculate concentration from peak area in HPLC analysis, you can use the formula: Concentration Peak Area / (Slope x Injection Volume). The peak area is obtained from the chromatogram, the slope is the calibration curve slope, and the injection volume is the volume of the sample injected into the HPLC system.
Why ordinary liquid pump cannot be used in hplc system?
An ordinary liquid pump cannot be used in HPLC systems because HPLC requires pumps that can generate high pressure to push solvents through the column at a constant and precise flow rate. Ordinary pumps do not provide the level of pressure and flow rate control needed for HPLC separations, which can negatively impact the accuracy and reproducibility of chromatographic results. HPLC pumps are specifically designed to handle the high pressures and flow rates required for efficient separations.
