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What is the Full form of pMB1 origin of replication?

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Anonymous

∙ 12y ago
Updated: 6/20/2024

The full form of pMB1 origin of replication is the originating origin of the wild-type plasmid minB.

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∙ 1y ago
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Why pBR322 plasmid have high copy number?

I think pBR322 has a replication module from E coli plasmid colE1 ,which permits plasmid replication even when chromosome replication and cell division are inhibited by amino acid starvation and chloramphenicol, as a result, under such condition each cell accumulates several thousands copies of the plasmids up to 3000, so that one litre of bacterial culture easily yields a milligram of plasmid DNA.


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Why pBR322 plasmid have high copy number?

I think pBR322 has a replication module from E coli plasmid colE1 ,which permits plasmid replication even when chromosome replication and cell division are inhibited by amino acid starvation and chloramphenicol, as a result, under such condition each cell accumulates several thousands copies of the plasmids up to 3000, so that one litre of bacterial culture easily yields a milligram of plasmid DNA.


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