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What is charge separation across the bacterial membrane?
charge separation is when the charge from an object is repelled by another obeject and the charge from that object is separated throughout the whole entire object.
Electrical charge resulting from the difference between positive and negative ions outside and inside the brain cell membrane is called?
Membrane potential
What is a nerve membrane at rest called?
When the neural membrane (neurolemma) is at rest it is said to have resting potential, polarized, and has a negative charge inside.
What is the small change in the charge across a neuron's membrane called?
Action Potential
What is blotting and types of blotting?
Blotting is a technique of transferring DNA or RNA or Protein from a gel to the membrane. nitrocellulose, PVDF or nylon membrane are used to attach these molecules permanently on them. Western, southern and norther blotting are the techniques used to transfer protein, DNA and RNA respectively. It is done to detect them with a specific probe or antibody. This can not be detected or easy to handle on the gel, so we do blot for these analysis.
What is the difference between nylon membrane and nitrocellulose membrane?
Nylon membranes are less brittle and easier to handle than nitrocellulose, making them ideal for reprobing. They also respond more robustly to various environmental storage conditions than nitrocellulose. Nylon's highly hydrophilic nature makes prewetting unnecessary, and nylon membranes have much higher binding capacities than nitrocellulose for nucleic acids.
How do you get nitrocellulose?
You can get nitrocellulose from playing cards and films if broken apart.
What is a membrane potential?
a voltage or electrical charge across the plasma membrane
How do you make nitrocellulose thinner what are the solvents and the ratio?
Nitrocellulose is soluble in a mixture of ethanol and ethylether.
What is the purpose of using the nylon membrane?
To transfer protein from a gel to a positively charged surface! that nylon or nitrocellulose membrane can be served as a plane for protein to be attached on it after western blotting
Does a cell membrane has a charge?
yes
What is true of ions and their transport across cell membranes?
electrical or chemical signals may control the movement of ions across cell membrane
What is the charge on the inside membrane during the resting membrane potential?
The resting membrane potential of a typical neuron is around -65mV
What is a sudden electrical charge in a neuron membrane is?
Yes.
What is in charge of what enters and leaves the cell?
The cell membrane
Is nitrocellulose present in red gum?
No.
What is charge separation across the bacterial membrane?
charge separation is when the charge from an object is repelled by another obeject and the charge from that object is separated throughout the whole entire object.
