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What is the purpose of the buffer in PCR?
The purpose of the buffer in PCR, I assume you talking about the 5 or 10 times PCR buffer that is provided with enzyme. Buffer is needed to give the correct pH and pottasium ion concentration for the DNA polymerase enzyme (usually DNA polymerase from Thermus aquaticus) to function.
If E. coli DNA polymerase was used instead of thermus aquaticus DNA polymerase in a pcr polymerase chain reaction procedure then will reaction be carried out in waterbath?
no e coli DNA pol can not with stand high temperature if so for every denaturation step we must add fresh enzyme
Why normal DNA polmerase not use in PCR?
In the PCR, high temperatures are used in order to separate both strands of DNA readily. Normal DNA polymerases would "melt" (denature) under these conditions, whereas Taq DNA Polymerase does not (short from Thermus aquaticus, a bacteria that lives in very hot submarine springs).
What are the selective media for isolation of thermophiles?
thermus agar
Why taq polymerase is thermostable?
Taq Polymerase is an important enzyme component involved in the PCR reaction. Its A DNA polymerase and its role is to elongate the growing strands of DNA during the extension process. Since the Extension process in a PCR works at a temperature which a human DNA polymerase cannot remain active, the Taq polymerase obtained from Thermus aquaticus (living in the hot springs) are used and hence these enzymes are thermo stable.
Which characteristic is seen in a mushroom but not in thermus aquaticus?
A mushroom is a eukaryote and a thermos aquaticus is a prokaryote
What is the disease of thermus aquaticus?
orangeatitus is the main disease.
5 microorganism of the desert biome?
Thermus aquaticus Thermus thermophilus Chloroflexus aurantiacus Pyrodictium abyssi
Where was Thermus Aquaticus found?
In yellowstone national park in 1969 by freeze and brock
Which charcteristic is seen in a mushroom but not thermus aquaticus?
Characteristics seen in a mushroom but not in thermus aquaticus include a cap and stipe, or stem. The major part of a mushroom is concealed beneath soil or within decomposing materials, such as rotting trees or leaves.
What has the author CM Menendez written?
C.M Menendez has written: 'Producation of the thermostable dna polymerbase from thermus aquaticus in escherichia coli'
What is the scientific name for extremophile?
Answer ThermophilesThere are several different types of thermophiles, each has its own name:Pyrococcus furiosusThermus aquaticusThermus thermophilusChloroflexus aurantiacusThermococcus litoralisPyrodictium abyssiBacillus stearothermophilusThermobifida fusca(The list is not final)Thermus aquaticus
What has the author Jorge Rivera-Cifuentes written?
Jorge Rivera-Cifuentes has written: 'Purification and properties of the prolyl-tRNA synthetase from 'thermus aquaticus''
What are some unicellular organisms?
Paramecium bursaria Toxoplasma gondii Crenarchaeota Korarchaeota Testate amoebae Escherichia coli Jonquetella Thermus aquaticus Leptomyxida
When was Asellus aquaticus created?
Asellus aquaticus was created in 1758.
What does tribulus aquaticus have to do with bodybuilding?
tribulus aquaticus contains steroidal glycosides.
What is the purpose of the buffer in PCR?
The purpose of the buffer in PCR, I assume you talking about the 5 or 10 times PCR buffer that is provided with enzyme. Buffer is needed to give the correct pH and pottasium ion concentration for the DNA polymerase enzyme (usually DNA polymerase from Thermus aquaticus) to function.
