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BL21 is RecA+, whereas DH5alpha is RecA- and thus more appropriate for cloning purposes. BL21 also produces lower levels of proteases and thus better for expression of recombinant proteins.

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What are the differences between a right-handed alpha helix and a left-handed alpha helix in protein structures?

The main difference between a right-handed alpha helix and a left-handed alpha helix in protein structures is the direction in which the helix twists. In a right-handed alpha helix, the helix twists in a clockwise direction, while in a left-handed alpha helix, the helix twists in a counterclockwise direction. This difference in twisting direction affects the overall shape and stability of the protein structure.


What is the difference between beta and alpha hemolysis in terms of their effects on red blood cells?

Beta hemolysis completely lyses red blood cells, causing a clear zone around the colony on blood agar. Alpha hemolysis partially lyses red blood cells, resulting in a greenish discoloration around the colony on blood agar.


What are the key characteristics and applications of the DH5 alpha strain in molecular biology research?

The DH5 alpha strain is commonly used in molecular biology research due to its high transformation efficiency, making it easy to introduce foreign DNA. It is also known for its ability to maintain plasmids well and its sensitivity to antibiotic selection. Researchers often use this strain for cloning, gene expression studies, and protein production.


Where are ATP high energy bonds located?

It is stored in phosphodiester bonds formed between alpha to gamma and beta to gamma.Please note that the bond between alpha to beta is not considered a high energy bond.


The coiling of the protein chain backbone into an alpha helix is referred to as?

The coiling of the protein chain backbone into an alpha helix is referred to as secondary structure. This repetitive structure is stabilized by hydrogen bonds between the backbone amide hydrogen and carbonyl oxygen atoms.

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What are the differences between a right-handed alpha helix and a left-handed alpha helix in protein structures?

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