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Prototrophs are the wild type cells and would be the bacterial cells capable of utilizing certain nutrients or synthesizing certain compounds. Auxotrophs would be the mutant cells that are not capable of utilizing certain nutrients or synthesizing certain compounds. In the example of bacteria capable of utilizing Lactose as a nutrient source, the ability to lactose is denoted by lac+ and the inability to utilize lactose is denoted lac-.

  • Prototrophs = lac+
  • Auxotrophs = lac-
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Q: What is the difference between prototrophs and auxotrophs?
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