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The Gram reaction is used to classify bacteria into two broad categories, Gram positive and Gram negative. The Gram stain characteristics of a bacterium depends on its cell wall components and thickness. The viruses do not have any cell walls or other equivalent. The viruses are too small to be seen by light microscopy, only exception is the Pox virus. So the Gram stain is not appropriate for virus identification. However, if one is able to see papilloma virus through light microscope, these may appear Gram negative. Because the crystal violet dye would be removed by decolorizer, and the counterstain (safranin or carbol fuchsin) would show its color.
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What are the gram reaction of mycobacterium?
Mycobacteria are typically Gram-positive, but their cell wall structure is unique and contains high lipid content, making them resistant to Gram staining. They are best visualized using acid-fast staining methods, such as the Ziehl-Neelsen or Kinyoun stains.
What might cause a Gram variable reaction?
A Gram-variable reaction may occur when the bacteria have an atypical cell wall structure, making it difficult to predict whether they will appear as Gram-positive or Gram-negative under a microscope. This variability in staining can be caused by factors such as age of the culture, cell wall composition, or bacterial species.
What does a gram reaction of a cell refer to?
the Gram reaction is based on the structure of the bacterial cell wall. In Gram-positive bacteria, the dark purple crystal violet stain is retained by the thick layer of peptidoglycan which forms the outer layer of the cell. In Gram-negative bacteria, the thin peptidoglycan layer in the periplasm does not retain the dark stain, and the pink safranin counter stain stains the peptidoglycan layer. In other word,the gram reaction refers to how the cells reacts to the gram-staining process.
What is the result of gram stain on human cell?
Human cells have no cell walls, only a plasma membrane, and as a result lack the peptidoglycan layer that gram stains utilize to differentiate between gram negative and gram positive species of bacteria. Therefore, human cells are unable to retain the crystal violet introduced in the first step of the gram stain, and stain negative. (they appear pinkish like gram-negative bacteria)
What is the weight of a human embryo?
The weight of a human embryo can vary, but on average, it is around 0.1 gram at 6 weeks of development.
What are some of the reason for a Gram variable reaction?
what are some of the reasons for a gram-variable reaction
What gram reaction do you expect from acid-fast bacteria?
Gram positive
What is the gram stain reaction of strep throat?
Streptococcus pyogenes is gram positive bacteria.
What are the factors that can result in variable gram reaction?
Genetics, Age of culture, type of growth medium, and technique used could result in a gram-variable reaction
Does a bacteriums gram reaction seem to be directly related to its shape?
Yes, a bacterium's gram reaction is often related to its shape. Gram-positive bacteria have a thick peptidoglycan layer that retains the crystal violet dye used in the Gram staining procedure, while gram-negative bacteria have a thinner peptidoglycan layer and an outer membrane that does not retain the dye. The shape of the bacterium can influence how well the dye penetrates and stains the cell wall, affecting the outcome of the Gram reaction.
What are the gram reaction of mycobacterium?
Mycobacteria are typically Gram-positive, but their cell wall structure is unique and contains high lipid content, making them resistant to Gram staining. They are best visualized using acid-fast staining methods, such as the Ziehl-Neelsen or Kinyoun stains.
What might cause a Gram variable reaction?
A Gram-variable reaction may occur when the bacteria have an atypical cell wall structure, making it difficult to predict whether they will appear as Gram-positive or Gram-negative under a microscope. This variability in staining can be caused by factors such as age of the culture, cell wall composition, or bacterial species.
What does a gram reaction of a cell refer to?
the Gram reaction is based on the structure of the bacterial cell wall. In Gram-positive bacteria, the dark purple crystal violet stain is retained by the thick layer of peptidoglycan which forms the outer layer of the cell. In Gram-negative bacteria, the thin peptidoglycan layer in the periplasm does not retain the dark stain, and the pink safranin counter stain stains the peptidoglycan layer. In other word,the gram reaction refers to how the cells reacts to the gram-staining process.
Are human cells gram positive?
Human cells are Gram-negative because they do not contain certain structures. More simply, if they don't have a cell wall they cannot be Gram-positive.
What is the gram reaction of s epidermidis?
Staphylococcus epidermidis is a Gram-positive bacterium, meaning it retains the crystal violet stain in the Gram staining procedure. This results in a purple color under the microscope.
What is the result of gram stain on human cell?
Human cells have no cell walls, only a plasma membrane, and as a result lack the peptidoglycan layer that gram stains utilize to differentiate between gram negative and gram positive species of bacteria. Therefore, human cells are unable to retain the crystal violet introduced in the first step of the gram stain, and stain negative. (they appear pinkish like gram-negative bacteria)
How many gram of blood in human body?
There are .000373 grams of blood in an average human body.
