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The two strands are held in position by hydrogen bonding between the bases.

All the way along a DNA molecule, every base on one strand is paired with a base on the other strand. This pairing follows a "rule" of complementary base pairing, by which each base can only be paired with one of the other three (A with T, C with G).

The hydrogen bonding is not as strong as covalent bonds. At about 94 degrees C, the two strands separate as heat ruptures the hydrogen bonds.

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