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I'm not sure about Tryptic Soya Agar but where I work we regularly use Tryptic Soya Broths (TSB). All our samples are human tissue samples and they are placed in a small bottle containing the TSB solution. These are then sent for testing. So I imagine Tryptic Soya Agar would be used for similar purposes except of course it would be a solid jelly in a dish and would have to be swabbed with the sample. They're basically just food for any bacteria or fungi that are present.
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Is it possible to melt a tryptic soy agar slant to make a tryptic soy broth?
No. Agar is a solid at room temperature and would just become solid again. A TSB does not contain agar and therefore can be liquid at room temperature.
What does Proteus vulgaris look like on a Tryptic Soy Agar media?
It swarms the whole plate.
Why is it important that the Tryptic Soy Agar slant be inoculated with a pure bacterial culture instead of a mixed culture?
So that you can be sure that the results are due only to one specific species.
Why is Tryptic soy agar considered a complex medium?
It's complex because the constituent elements of the medium (enzymatic digests of casein and soybean) are present in an undefined amount, and an amount which will vary from batch to batch. Contrast this to a chemically defined medium which will always contain the exact amount of constituent.
A common medium used for growing fastidious bacteria is?
Blood Agar, and trypticase soy agar grows semi fastidious
Is E. coli white or buff color on tryptic soy agar?
E. coli is actually green on tryptic soy agar. The agar itself is a white, amber color.
Is it possible to melt a tryptic soy agar slant to make a tryptic soy broth?
No. Agar is a solid at room temperature and would just become solid again. A TSB does not contain agar and therefore can be liquid at room temperature.
What does Proteus vulgaris look like on a Tryptic Soy Agar media?
It swarms the whole plate.
How long does it take non pathogenic Serratia Marcescens D1 to grow and can you use nutrient agar as a medium?
Two days at room temperature on TSA (tryptic soy agar)
Why is it important that the Tryptic Soy Agar slant be inoculated with a pure bacterial culture instead of a mixed culture?
So that you can be sure that the results are due only to one specific species.
Why is a TSA used for the streak plate?
Tryptic soya agar plates are used for streaking as it is a very general agar (ie not selective) and it promotes the growth of a broad spectrum of micoorganisms
Why is Tryptic soy agar considered a complex medium?
It's complex because the constituent elements of the medium (enzymatic digests of casein and soybean) are present in an undefined amount, and an amount which will vary from batch to batch. Contrast this to a chemically defined medium which will always contain the exact amount of constituent.
A common medium used for growing fastidious bacteria is?
Blood Agar, and trypticase soy agar grows semi fastidious
How do you isolate staphylococcus aureus from hospital floor?
Use a moistened sterile swab to sample the floor. Put this is tryptic soy broth and incubate for 24 hrs at 32 deg C. Streak the resulting solution on to mannitol soy agar and incubate at 32 deg C until colonies form.
What is TSA in microbiology?
Trypticase soy agar
A bacterial species that grows on blood agar but will not grow on trypticase soy agar is termed an anaerobe?
false
Is a blood agar selective or differential?
Blood agar is a bacterial growth medium which contains 5% sheep's blood. It is considered to be differential but not selective, because it is an enriched medium that provides a rich nutrient environment for many types of bacteria, while a selective medium supports the growth of certain types of bacteria but inhibits other types. Blood agar is considered differential because it is used to distinguish pathogenic bacteria based on the effect of bacterial enzymes known as hemolysins which lyse red blood cells. Blood agar is mainly used clinically to detect the presence of Streptococcus pyogenes, the human pathogen which causes "strep throat". Blood agar can be made selective by the addition of other ingredients.
