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A zone of clearing is an area on an agar plate in which there is no microbial growth due to the presence of an inhibitory substance. Antibiotics placed on an agar plate will induce a zone of clearing if the bacteria it is being tested against is susceptible. Zones of clearing can also be created by other kinds of bacteria which produce exozymes or antibiotics themselves into the media to inhibit the growth of competing bacteria. Streptomyces are antibiotic producers who can be found in soil.

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The zone of inhibition in microbiology experiments is measured by using a ruler to determine the diameter of the clear area around an antimicrobial disk where bacteria do not grow. This measurement helps determine the effectiveness of the antimicrobial agent against the bacteria being tested.


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To accurately measure the zone of inhibition in a microbiology experiment, use a ruler to measure the diameter of the clear area around the antibiotic disk where bacterial growth is inhibited. Take measurements from multiple points and calculate the average to get a more precise result.


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