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Despite the initial success of van Leeuwenhoek's relationship with the Royal Society, this relationship was soon severely strained. In 1676 his credibility was questioned when he sent the Royal Society a copy of his first observations of microscopic life forms.

In 1676 his credibility was questioned when he sent the Royal Society a copy of his first observations of microscopic life forms. In an experiment he then discovered "wee animals" in the water-so small that if one hundred were laid end to end "they could not reach to the length of a grain of coarse sand." The existence of such life forms was entirely unknown an even with his established reputation with the Royal Society as a reliable observer, his observations of microscopic life were initially questioned. Although the Royal Society doubted van Leeuwenhoek's discoveries he continued insist to the Royal Society that his observations were accurate, true, and valid.

Eventually, in the face of van Leeuwenhoek's insistence, the Royal Society arranged to send an English vicar, as well as a team of respected jurists and doctors to Delft to determine whether Leeuwenhoek's observations were indeed true. In 1680, van Leeuwenhoek's observations were fully vindicated by the Society.

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