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What else can I help you with?
Explain why crystal violet would not be useful in the negative stain technique?
Negative staining techniques are designed to stain everything BUT the bacterial cells. This allows us to see the cells unstained and helps us observe their morphology (how the cells are shaped and how they group together). The cells have an overall negative charge on their surface, so they naturally attract positive charges. Crystal Violet carries a positive charge, so it would adhere to the surfaces of the cells thus staining the cells (which is not what you want in a negative stain!)
What color are bacterial endospores after a gram stain is performed?
Gram-staining does not stain the endospore due to the tough, resistant water-proof structure. It appears as an unstained area in a vegetative cell. Malachite green must be forced into the endospore with heat to stain it.
What is a particular organism or part of a organism being studied called?
Specimen
Why would a CBC specimen be rejected?
A CBC specimen may be rejected due to specimen hemolysis (breakdown of red blood cells), inadequate sample volume, clotted specimen, improper handling or storage, or outdated sample. These factors can affect the accuracy and reliability of the test results.
Why is the gram stain technique more valuable than the simple stain technique in the diagnostic laboratory Under what circumstances might the simple stain be preferable to the gram stain technique?
There are two types of stains, the simple stain and the differential stain. A simple stain colors all objects the same while a differential stain is used to spot differences in microorganisms. A gram stain is a differential stain, which is used to tell the difference in gram negative and gram positive bacteria. A simple stain would stain all the organisms the same and this difference would not be noted. You would be able to determine their shape, whether it is a cocci or bacillus (rod), but not the type. I'm not sure why the simple stain would be preferable unless you just wanted a quick answer as to the shape of the bacteria. In some cases, a wet prep can be made of a presumptive gram positive cocci to tell the difference between bacteria or yeast. Otherwise, I would say that the gram stain is the only way to go.
What would you use to hold a patient tongue down when a specimen is obtained for a throat culture?
Forceps
What stain would you select if asked to evaluate a patient's bone marrow smear for the presence of siderosomes?
new methylene blue
How would your slide (cells and endospores) have appeared if you had performed a Gram stain rather than an endospore stain?
If a Gram stain had been performed instead of an endospore stain, the slide would show the bacterial cells either as purple (Gram-positive) or pink (Gram-negative) based on their cell wall composition. The endospores, which typically do not retain the Gram stain, would likely appear as clear or colorless structures within the stained cells. Thus, the focus would be on the overall morphology and arrangement of the vegetative cells rather than highlighting the presence of endospores.
Explain why crystal violet would not be useful in the negative stain technique?
Negative staining techniques are designed to stain everything BUT the bacterial cells. This allows us to see the cells unstained and helps us observe their morphology (how the cells are shaped and how they group together). The cells have an overall negative charge on their surface, so they naturally attract positive charges. Crystal Violet carries a positive charge, so it would adhere to the surfaces of the cells thus staining the cells (which is not what you want in a negative stain!)
What color are bacterial endospores after a gram stain is performed?
Gram-staining does not stain the endospore due to the tough, resistant water-proof structure. It appears as an unstained area in a vegetative cell. Malachite green must be forced into the endospore with heat to stain it.
What would you see if you observe a specimen with a magnifying glass?
Obviously, an enlarged specimen.
How big would the specimen look?
the specimen is the objective the microscope and a parts of microscope
What is a particular organism or part of a organism being studied called?
Specimen
When would it be acceptable to collect a urine specimen from the urine drainage bag?
Collecting a urine specimen from a urine drainage bag is acceptable when it is necessary to obtain a sample for laboratory analysis, and no other options are available, such as a clean catch. However, it should be done with caution to avoid contamination, and the specimen should be collected from the sampling port rather than the bag itself. It is important to ensure the sample is fresh and representative of the patient’s urine output. In general practice, obtaining a sample directly from the patient is preferred when possible.
If applying a green stain over a red stain what colour would I get?
You would get the color grey
A sentence with specimen?
A sentence with the word specimen in it would be, I went to the lab today to pick up that specimen I dropped off to be studied last week.
What would happen if no fixing were done?
If no heat fixing was done to a slide with a specimen on it, it would be rinsed off with the gram staining procedure. Heat fixing the specimen does kill specimen but it also locks it in place.
