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Five I's

1. Inoculation: The sample is placed into a container of sterile medium that provides microbes with the appropriate nutrients to sustain growth.

2. Incubation: An incubator can be used to adjust the proper growth conditions of a sample.

3. Isolation: The end result of inoculation and incubation is isolation of the microbe.

4. Inspection: The cultures are observed for obvious growth characteristics that could be useful in analyzing the specimen contents.

5. Identification: Determine the type of microbe, usually to the level of species.

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13y ago

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