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Genes in eukaryotic cells are in three places: the nucleus, mitochondria, and chloroplasts.

A gene is a segment of DNA that codes for a polypeptide or for a molecule of RNA, such as a transfer RNA (tRNA) or ribosomal RNA (rRNA). The genes in the nucleus are in chromosomes. A chromosome consists of a molecule of DNA (two after replication of the DNA) and associated proteins.

Genes are segments of DNA molecules. A single DNA molecule has numerous genes. For example, humans have 23 chromosomes and about 20,000 to 25,000 genes in a haploid set, so there are, very roughly, a thousand genes on an average chromosome, which is to say along an average molecule of DNA. Genes in mitochondria and chloroplasts are on loops of DNA, like prokaryotic chromosomes. Mitochondria have 37 genes, some of which encode polypeptides, while the others code for rRNA and tRNAs. Chloroplasts have more than this (over 120 in one liverwort).

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