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Where can you find the genomic and transcript sequences of the gene PKD1 and the amino acid sequence of the protein polycystin-1?
Wiki User
∙ 15y ago
Try the NCBI (National Center for Biotechnology Information) site. On the home page (there is a link below) enter PKD1 in the search box.
A good website for human (and other) gene locations and sequences is Ensembl. Try, for example, clicking the link below, and use the left side-bar, or scroll down for the transcript sequence.
All information on the Ensembl website is in the public domain.
There is relevant discussion in Cell 1995 Apr 21; 81(2):289-9. See the Cell Press link below for a PDF of the Cell article.
Wiki User
∙ 15y ago
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What is the difference between genomic and bioinformatics?
genomics is the study of an entire sequence of an organism's DNA, while bioinformatics is the use of computers and data bases to organize and analyze DNA. bioinformatics makes genomics a bit easier.
How is a genomic library produced?
A genomic library is produced through the meticulous accumulation of genetic information that lends to different classifications and structures to the level of taxonomy.
What is the difference between animal and plant DNA extraction?
Plant DNA extractionPlant genomic DNA is more difficult to extract because of the plant's cell wall, which is removed by homogenization, or by adding cellulase to degrade the cellulose that makes up the cell wall. Also, the metabolites present in the plant cell may interfere with genomic DNA extraction by contaminating the DNA sample during the precipitation process.Animal DNA ExtractionPeripheral blood leukocytes are a main source of animal genomic DNA, but sample collection is difficult as blood must be withdrawn from the animal. Blood contains a range of compounds like proteins, lipids, white blood cells, red blood cells, platelets, and plasma, which can contaminate the DNA sample. The primary contaminant of animal DNA extracted from blood samples is heme, the non-protein component of hemoglobin.DifferencesThe differences between plant and animal DNA lie in the sequence of bases in the helix. Compounds found in plant cells are absent in animal cells, and DNA base sequences reflect this, as the genomic plant DNA is often larger than animal DNA. These differences affect extraction methods, as it impacts on yield and purity of DNA.
What phenomenon occurs when a particular alelle will either be expressed or silenced depending on whether it is inherited from a male or female?
genomic imprinting is the answer to the question above....found in my text book...
Applications of chromosome walking and jumping?
You want to know the sequence of a gene and only know the sequence of 2 DNA markers near it such as an RFLP (stands for Restriction fragment length polymorph) or another gene. Using different restriction enzymes, cut the genome of the organism to create DNA clones which overlap. For example, if the genome is ABCDEFGHI, enzyme 1 cuts it into 2 pieces to make ABCD EFGHI and enzyme 2 cuts it into 3 pieces to make ABC DEF GHI.. These pieces will over lap in sequence because the enzymes have different restriction sites. Using the known sequence of the RFLP, a radio labeled nucleic acid probe can be made to search the genomic library. The probe will anneal to clones with overlaps, and the ends of these clones can be made into new probes to search for the adjacent sequence. This is repeated until the desired segment has been cloned. each clone is sequenced and the overlaps are compared to determine the order of the sequences. This technique can be used to analysis diseases, such as cystic fibrosis, to look for mutations.
How can you estimate the size of the genomic library you want and need to develop to be sure that a particular gene is present in that library?
probability of including any DNA sequence in a random libray of N independent recombinants: N= [ln(1-P)] / [ln(1-1/n)] where n= genome size /average insert size in theory, a genomic library should contain all the different sequences, but in practice, a genomic libray is never perfect.
Does a restriction enzyme generate the same size fragments in genomic DNA of different species?
No. A restriction enzyme cuts DNA when it finds a specific sequence. Different animals will have these sequences occur at different intervals so the length of the fragments won't be the same.
Are blue eyes a mutation?
No. Mutations are changes in a genomic sequence. Blue eyes are not changes.
What is a genomic library?
Specific human genes stored in virus, bacteria and yeast hosts no, the genes are randomly inserted into vectors. a cDNA library houses tissue-specific sequences derived from an mRNA transcript so that it contains only genes that code for protein.
What did Haseltine begin patenting?
human genomic sequences that had the potential for medical use. He formed partnerships with major pharmaceutical companies and garnered a reputation for giving brilliant presentations to venture capitalists.
Scientists use PCR primarily to?
PCR stands for Polymerase Chain Reaction. It is a process used to take a small piece of DNA and use it to reproduce copies of a particular genomic sequence. This is useful in testing for genetic diseases, forensic science, and paternity testing.
Why you chose mRNA rather than genomic DNA for making a DNA library?
Complementary DNA (cDNA) is a doublestranded DNA version of RNA . Messenger RNA is a more useful predictor of a polypeptide sequence than DNA, because the introns have been spliced out. Scientists use cDNA rather than mRNA itself because RNAs are less stable than DNA.
When was Genomic Standards Consortium created?
Genomic Standards Consortium was created in 2005.
When was Genomic Medicine Institute created?
Genomic Medicine Institute was created in 2005.
What is a map of the distribution of cloned genomic DNA from genomic clone libraries?
physical map
What is genomic chips?
Genome chips are miniaturized plates containing hundreds of microscopic wells on their surface. These wells contain DNA probes. DNA probes are basically stretches of cDNA from a particular genome. When genomic DNA isolated from an organism is allowed to interact with the cDNA probes, come probes bind to the genomic DNA while others do not (depending on complementarity. A laser light is used to read each well and look at what sequences are bound. This information is valuable to scientists who can determine changes in gene expression based on the information obtained from a gene chip
What is the difference between genomic and bioinformatics?
genomics is the study of an entire sequence of an organism's DNA, while bioinformatics is the use of computers and data bases to organize and analyze DNA. bioinformatics makes genomics a bit easier.
