Which selective medium is used for Trichoderma viridea to grow?

Answer 1

No medium exists for the selective growth of particular Trichoderma species, i.e. unless a pure spore culture is used of T. viride, one won't be able to select for it specifically using growth media. You can however, use a Trichoderma Selective Medium (TSM) to suppress growth of other fungi/bacteria and obtain Trichoderma colonies that can then be differentiated by morphology. Once a pure culture is obtained, subs can then be made onto various media such as potato dextrose agar (PDA), malt extract agar (MEA) on which Trichoderma will happily grow profusely and sporulate. TSM suppresses fungal growth making Trichoderma colonies grow very slowly, so this not a good media to use if sporulation is the objective.

Answer 2

0 2 g MgSO4 (7H:O) (Unilab), 0 9 g

K:HPO, (Univar), 015 g KCl (Unilab), 10 g

N H4NO3 (Univar),30gD(-(- )glucose anhydrous

(Univar), 0 15 g rose bengal (BDH Chemicals)

and 20 g agar (Merck). These constituents were

added to 950 ml of distilled water and autoclaved

at 121 C for 15 min. The biocidal ingredients

were 0 25 g chloramphenicol (crystallized)

(Boehringer Mannheim), 02 g quintozene

(PCNB. wettable powder, 750 g/kg a.i.) (May Baker), 0-2 g captan (Kaptan 517, wettable

powder, 500 g/kg a.i.) (AECI) and either propamocarb-

hydrochloride (Previcur N-12, solution

concentrate, 722 g/l a.i.) (Schering International)

or metalaxyl (Ridomil 273, granular, 50 g/kg a.i.)

(Ciba Geigy) at 1-2 m) and 1-6 g respectively.

These were mixed in 50 ml of sterilized (autoclaved

at 121 C for 15 min) distilled water and

added to the autoclaved basal medium. Thus,

except for propamocarb or metalaxyl (chosen for

anti-oomycete activity) replacing fenaminosulf,

this medium was identical to that of Elad & Chet

(1983).