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Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?
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∙ 6y ago
Differential
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∙ 6y ago
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Why is a solid medium used to purify microorganisms?
Solid medium is used to purify microorganisms so that the microorganism can develop pure colonies quickly. The most commonly used solid medium is agar.
What are a group of microorganisms called?
In the broadest terms, a group of microorganisms can be an ecosystem of its own, or a population. For example the group of microorganisms that inhabit your mouth are the population of your oral cavity. A species is every organism that exhibits the same DNA (within reason, there are so many ways DNA can be mutated) and key features. The most often time you see a group of microorganisms may be on a agar plate, after incubating the plate you've streaked with bacteria. In this case the groups, or blobs, are called 'colonies'. While each colony has millions(!!) of bacterial cells in it (hence why you can see it!), they all came from one bacteria. Because we know this we can count the number of colonies and know how many bacteria were originally on the plate, somethings microbiologists call CFU, or Colony-Forming-Units. Many examples in food and beverage industry involved maximum CFU values per mL of beverage or gram of food for it to pass inspection. This is how it is determined.
What are the Different methods used to isolate microorganisms?
1 The Spread Plate: If a mixture of cells is spread out on an agar surface so that every cell grows into a completely separate colony, a macroscopically visible growth or cluster of microorganisms on a solid medium, each colony represents a pure culture. The spread plate is an easy, direct way of achieving this 2 The Pour Plate: Extensively used with bacteria and fungi, a pour plate also can yield isolated colonies. The original sample is diluted several times to reduce the microbial population sufficiently to obtain separate colonies when plating result. 3 The streak plate: Pure colonies also can be obtained from streak plates. The microbial mixture is transferred to the edge of an agar plate with an inoculating loop or swab and then streaked out over the surface in several patterns
Why is it important to start a bacterial culture with a single isolated colony?
It is important for someone who works with bacteria to have a genetically identical bacterial colony so they can test different things on one type of bacteria. Making observations on that one colony is valuable for visual research and identification of that microorganism.
How would you determine whether a colony was a contaminant on a streak plate?
One to determine whether a colony on a streak plate is a contaminant is by observing whether it is located along the streak lines. Another is to compare the size, shape, texture and color of the colony against an uncontaminated sample to see if it matches previous ones. Anything growing beyond streak lines and outside of the expected pattern of growth is an obvious contaminant.
How would you determine whether a colony was a contaminent on a pour plate?
The contaminant colony will vary in shape, so the appearance of colonies can be used to distinguish different bacteria.
What is the primary use of slants?
They are used to culture microorganisms and provide greater surface area for the growth of colonies.
Why is a solid medium used to purify microorganisms?
Solid medium is used to purify microorganisms so that the microorganism can develop pure colonies quickly. The most commonly used solid medium is agar.
Distinguish between multicellular organisms and colonies of unicellular organisms?
protists, plants, animals, and fung.
Distinguish characteristics of the middle colonies?
Each colony had its own unique characteristics, but historians lump them. The people who founded the Middle Colonies were looking to practice their own.
Why is the total viable count based on 30-300 colonies of microorganisms?
this number is chosen as it is high enough to have statistical accuracy, yet low enough to avoid nutrient competition among developing colonies.
What micro organisms cant you see?
All of them. "Micro" means "tiny". You need a microscope to see them. Colonies of microorganisms might be visible, but not an individual.
What different types of colonies were developed in the New World?
Proprietary colonies, royal colonies, and charter colonies.
What determines the size of cultured colonies?
The size of the cultured colonies is usually determined by how densely the plate is populated. The more densely populated the plate, the greater the competition is among the microorganisms for nutrients. This competition results in the growth of reletively small colonies which tend to be merged together. On a more sparsely populated plate, there are enough nutrients for the microorganisms to grow sufficiently resulting in larger colonies.
The most important economic enterprise in the colonies was?
The most important enterprises were different in separate colonies. Agriculture empires were the most important and different colonies farmed different crops and traded with the others.
How were the middle colonies different from the England colonies?
They WERE English, so no difference.
What made Carolina's different from other colonies?
theyre royal colonies
