The scientists Alfred Hershey and Martha Chase conducted a series of experiments at the Carnegie Institute of Washington in 1952, and concluded that the genetic material of a bacteriophage was DNA.
Hershey and Chase
Hersey and chase
Hershey and Chase radioactively labelled T2 bacteriophage with P (DNA) and S (protein) to determine if protein or DNA carried the genetic material. They let the bacteriophage infect E.coli. The radioactive material found in the host E.coli contained the radioactive P (found in DNA) as the bacteriophage had multiplied inside the cell, but the radioactive S was not found inside the cell. Therefore DNA is the hereditary molecule resorce - some other guy off the internet
DNA is the genetic material. However, Hershey-Chase also stated that some little sulfur containing material also entered the bacterial cell. Looking at these two conclusions, Hershey-Chase could not reach a definite conclusion as to whether DNA or Protein contains the genetic material. The conclusion came from Watson-Crick who stated that the sulfur containing material could have entered the cell due to the fact that DNA replication and subsequently translation of the mRNA leads to the synthesis of sulfur containing protein which were found in the bacterial cell.
It's genetic material and some enzymes sometimes which hijack the bacteria into making more viruses.
Bacteriophage.
Bacteria transfer DNA with a bacteriophage.
dna(:
they concluded that the genetic material of the bacteriophage was DNA, not protein
Hershey and Chase radioactively labelled T2 bacteriophage with P (DNA) and S (protein) to determine if protein or DNA carried the genetic material. They let the bacteriophage infect E.coli. The radioactive material found in the host E.coli contained the radioactive P (found in DNA) as the bacteriophage had multiplied inside the cell, but the radioactive S was not found inside the cell. Therefore DNA is the hereditary molecule resorce - some other guy off the internet
In their experiments, Hershey and Chase showed that when bacteriophages, which are composed of DNA and protein, infect bacteria, their DNA enters the host bacterial cell, but most of their protein does not. Although the results were not conclusive, and Hershey and Chase were cautious in their interpretation, previous, contemporaneous and subsequent discoveries all served to prove that DNA is the hereditary material.
DNA
A bacteriophage ("eater of bacteria") inject their DNA (or RNA) into the cell.
bacteriophage is a virus that kills bacteria
We can insert about 5-25 kb sized foreign DNA in a lambda bacteriophage vector.
Bacteriophage viruses are nonliving. They are DNA or RNA encapsulated in a protein coated capsule with recognition sites to bind to specific target sites on bacteria and insert their genetic material for viral replication or to induce changes in the bacterial genetic material.
DNA is the genetic material. However, Hershey-Chase also stated that some little sulfur containing material also entered the bacterial cell. Looking at these two conclusions, Hershey-Chase could not reach a definite conclusion as to whether DNA or Protein contains the genetic material. The conclusion came from Watson-Crick who stated that the sulfur containing material could have entered the cell due to the fact that DNA replication and subsequently translation of the mRNA leads to the synthesis of sulfur containing protein which were found in the bacterial cell.
Head region
Bacteriophage T4 virus