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Antonie van Leeuwenhoek was the first to see actual living things in water well over 300 years ago, in 1674. He saw moving animalcules or 'little animals' that were the algae Spirogyra and probably would also have seen bacteria in the sample.

A few years earlier in 1665 Robert Hooke had seen under a microscope non-living cell walls from studying slices of cork. This was a scientific achievement for the time, but he did not observe living things.

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