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Because a colony on plate could be formed by more than one cell.
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∙ 14y ago
Direct microscopy counts viable and non-viable cells, whereas plate count only counts viable cells that are able to grow and form colonies on agar plates. Additionally, plate count may underestimate the total number of viable cells due to factors like the inability of certain cell types to grow under specific conditions or the formation of aggregated cells that do not separate easily on the agar plate.
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What is the standard for aerobic plate count?
The standard for aerobic plate count, also known as aerobic colony count or Total Viable Count (TVC), is typically expressed in colony-forming units per milliliter (CFU/ml) or per gram (CFU/g) of sample. The acceptable limits can vary depending on the type of product or industry, but generally, lower counts indicate better hygiene and quality of the sample.
What is the difference between standard plate count and total plate count?
Standard plate count refers to the number of viable microorganisms in a sample that can grow under specific laboratory conditions, usually aerobic and mesophilic conditions. Total plate count includes all viable microorganisms, both aerobes and anaerobes, at any growth conditions.
Do both the spread-plate and pour-plate method in a experiment produce similar bacterial counts or are they vastly different?
The spread-plate and pour-plate methods generally produce similar bacterial counts if performed correctly. However, the spread-plate method may result in slightly lower counts due to potential bacterial loss during spreading, while the pour-plate method can sometimes lead to higher counts due to bacterial trapping within the agar. Overall, the difference in counts between the two methods is usually not significant.
What are the sources of error when performing a viable plate count?
Sources of error in viable plate counting include inaccurate dilutions leading to over or underestimation of colony forming units, uneven distribution of bacteria on the agar plate causing inaccurate colony counts, contamination from environmental sources impacting the results, and variability in the incubation conditions affecting bacterial growth rates.
Why is the viable plate count technique considered to be an indirect measurement of cell density whereas the turbidimetry method is not a count at all?
The viable plate count technique measures only the cells that are able to grow and form visible colonies on a solid agar medium, providing an estimate of viable cells present. In contrast, turbidimetry measures the amount of light passing through a liquid sample, which is influenced by various factors besides cell density. Since turbidimetry does not provide a direct count of cells, it is considered an indirect method for estimating cell density.
What causes high Aerobic plate counts in food?
inappropriete cleaning and sanitation of equipments and utensil may cause high aerobic plate count.
How do you compute colony forming unit per plate?
1.Direct Microscopic Counts (DMC) for both viable and nonviable cells
How do you measure bacteria in a petri dish?
When bacteria is grown in an Agar plate, one quantitative method to measure growth is using a counting chamber. Another method is using viable plate counts.
What does not counts as a plate appearance in baseball?
When you do not go to the bat.
What is the standard for aerobic plate count?
The standard for aerobic plate count, also known as aerobic colony count or Total Viable Count (TVC), is typically expressed in colony-forming units per milliliter (CFU/ml) or per gram (CFU/g) of sample. The acceptable limits can vary depending on the type of product or industry, but generally, lower counts indicate better hygiene and quality of the sample.
What if your instructor asks you to determine the number of organisms in a water sample observation of your dilution plates reveals?
I think you would eliminate plate counts that are not between 30-300 colonies. <30, because its too few to count, >300 too numerous to count.
What is the difference between standard plate count and total plate count?
Standard plate count refers to the number of viable microorganisms in a sample that can grow under specific laboratory conditions, usually aerobic and mesophilic conditions. Total plate count includes all viable microorganisms, both aerobes and anaerobes, at any growth conditions.
Do both the spread-plate and pour-plate method in a experiment produce similar bacterial counts or are they vastly different?
The spread-plate and pour-plate methods generally produce similar bacterial counts if performed correctly. However, the spread-plate method may result in slightly lower counts due to potential bacterial loss during spreading, while the pour-plate method can sometimes lead to higher counts due to bacterial trapping within the agar. Overall, the difference in counts between the two methods is usually not significant.
When does an at bat not count?
Hit by the pitch, sacrifice, base on balls and I believe catcher's interference
There are other techniques for counting bacteria such as a direct microscopic count and turbidity Why is the standard plate count preferrend for food?
The standard plate count method is preferred for counting bacteria in food because it provides a simple and reliable way to determine the number of viable bacteria present. It allows for the cultivation of various types of bacteria which may be present in the sample, providing a more accurate representation of the bacterial population. Additionally, the standard plate count method is well-established, widely accepted, and can be easily standardized for regulatory purposes in the food industry.
How do i read Aerobic plate count?
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If in baseball you're up at bat and you get walked with bases loaded and a run is forced in how can you get an RBI when a walk doesn't count as an 'at bat'?
It still counts as a plate appearance, and as a result of your plate appearance (bases loaded walk), a run scored. Therefore you are credited with an RBI. A sacrifice fly doesn't count as an at-bat either, but RBIs are credited. Double-plays are counted as at-bats but they disqualify RBIs. "At-bats" have absolutely nothing to do with RBIs.
