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The Gram positive bacterial cell wall is made up of thick peptidoglycan layer which is rich in Teichoic acids. These teichoic acids are negatively charged because of presence of phosphate in their structure.
The Gram negative bacteria have an outer membrane composed of phospholipids and Lipopolysaccharides. The lipopolysaccharides impart a strongly negative charge to surface of Gram negative bacterial cells.
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Why does nigrosin not stain bacterial cells?
Because Nigrosin is an acidic dye--carries a negative charge--and repels against the negatively charged cell walls of most bacteria.
What is the difference between acidic dye and basic dye?
basic dyes are more effective for bacterial staining than acidic dyes because basic dyes have a positive charged chromogen. Bacterial nucleic acids and certain cell wall components carry a negative charge that strongly binds to the cationic chromogen.
What part of bacterial cell is most involve with gram staining and why?
In the gram staining process, gram positive bacteria appear to be purple because their cell walls, which contain a large concentration of peptidoglycans, are strongly dyed. Gram negative bacteria appear pink because their walls asborb less dye. This occurs becayse there is a smaller concentration of peptidoglycans and an additional lipid layer surrounds the cell wall. ANSWER The exact mechanism of action of this staining technique is not clearly understood. However, it is known that differences in the biochemical composition of bacterial cell walls parallel differences in their Gram-stain reactions. Gram-positive bacterial walls are rich in tightly linked peptidoglycans (protein-sugar complexes) that enable cells to resist decolorization. Gram-negative bacterial walls have a high concentration of lipids (fats) that dissolve in the decolorizer (alcohol, acetone, or a mixture of these) and are washed away with the crystal violet. The decolorizer thus prepares gram-negative organisms for the counterstain.
Peptidoglycan is a unique macromolecule found in bacterial?
cell walls
Is there any similarity between bacterial cell wall and plant cell wall?
No, there is no similarity in bacterial cell walls and plant cell walls. They are different morphologically and also in chemical composition; plant cell walls are made up of cellulose, whereas bacterial cell walls are made up of peptidoglycan (also known as murein).
Why does nigrosin not stain bacterial cells?
Because Nigrosin is an acidic dye--carries a negative charge--and repels against the negatively charged cell walls of most bacteria.
Could a negatively charged dye stain a bacteria?
The effect on the bacteria depends if the stain is an acidic or basic stain. Most bacteria are stained when a basic stain permeates the cell wall and adheres by weak ionic bonds to the bacterial cell, which is slightly negatively charged.
Do bacterial plant and animal cells have walls?
only plant and bacterial cells have walls
What is the difference between acidic dye and basic dye?
basic dyes are more effective for bacterial staining than acidic dyes because basic dyes have a positive charged chromogen. Bacterial nucleic acids and certain cell wall components carry a negative charge that strongly binds to the cationic chromogen.
Soap can dissolve the outer walls of bacterial?
cells
Does bacterial wall contain chitin?
As far as I'm aware - no. Chitin is present in the cell walls of funghi but I believe bacterial cell walls are mostly made of proteoglycans.
What part of bacterial cell is most involve with gram staining and why?
In the gram staining process, gram positive bacteria appear to be purple because their cell walls, which contain a large concentration of peptidoglycans, are strongly dyed. Gram negative bacteria appear pink because their walls asborb less dye. This occurs becayse there is a smaller concentration of peptidoglycans and an additional lipid layer surrounds the cell wall. ANSWER The exact mechanism of action of this staining technique is not clearly understood. However, it is known that differences in the biochemical composition of bacterial cell walls parallel differences in their Gram-stain reactions. Gram-positive bacterial walls are rich in tightly linked peptidoglycans (protein-sugar complexes) that enable cells to resist decolorization. Gram-negative bacterial walls have a high concentration of lipids (fats) that dissolve in the decolorizer (alcohol, acetone, or a mixture of these) and are washed away with the crystal violet. The decolorizer thus prepares gram-negative organisms for the counterstain.
Peptidoglycan is a unique macromolecule found in bacterial?
cell walls
Large numbers of bacterial cells are not found in crown galls because?
cell walls protect the plant from bacterial invasion
Preparation made from killed bacteria or damaged particles from bacterial cell walls that can prevent some bacterial diseases?
Vaccine
Is there any similarity between bacterial cell wall and plant cell wall?
No, there is no similarity in bacterial cell walls and plant cell walls. They are different morphologically and also in chemical composition; plant cell walls are made up of cellulose, whereas bacterial cell walls are made up of peptidoglycan (also known as murein).
Bacterial cell walls contain a unique substance called?
chitin
