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Why is the E. coli lac Z gene used as a reporter gene in S. cerevisiae cells but not in E. coli cells?
The E. coli lac Z gene is used as a reporter gene in S. cerevisiae cells because the yeast lacks the lactose metabolism pathway, making it suitable for detecting lac Z expression. In contrast, E. coli naturally metabolizes lactose using the lac Z gene, so it is not ideal for use as a reporter gene in the same species.
How is the correct gene added to the cells?
The correct gene can be added to cells through a process called gene editing, where a specific gene is inserted into the cells using techniques like CRISPR-Cas9. This allows scientists to modify the genetic makeup of cells to introduce desired traits or correct genetic mutations.
What types of DNA sequences help eukaryotic cells regulate gene expression?
Eukaryotic cells use various DNA sequences to regulate gene expression, including promoters, enhancers, and silencers. Promoters are located near the start of a gene and initiate transcription. Enhancers and silencers can be distant from the gene and help to enhance or repress gene expression, respectively. Together, these sequences play important roles in controlling when and how genes are expressed in eukaryotic cells.
Does gene therapy use RNA?
Yes, some gene therapy approaches use RNA molecules to deliver therapeutic genetic material into cells. This can be done using techniques such as RNA interference (RNAi) or RNA-based viral vectors to introduce desired genetic changes into a patient's cells.
Which choice describes the process scientists use to mimic a natural genetic process of cells?
Scientists use techniques such as gene editing using CRISPR-Cas9 or gene targeting to mimic natural genetic processes in cells. These techniques allow scientists to introduce specific changes to the DNA of cells, similar to how mutations or genetic recombination occur naturally.
What code is use for protein?
gene
How does gene therapy aim to cure muscular dystrophy?
introduces good copies of the dystrophin gene into muscle cells. The goal is to allow the existing muscle cells to use the new gene to produce the dystrophin it cannot make with its flawed gene
What do Gene therapy researchers use to alter defective hereditary material?
Cells
What are cells DNA?
It's the code that cells use to construct the proteins that it needs.
What do you now have to do to successfully use your cloned gene to transform mice?
To transform the mice, you can: 1. inject the gene into the cells with microscopically small needles 2. use electroporation (an electical pulse that creates temporary pores in the plasma membranes of the cells that DNA can go through) 3. use a powerful virus
A gene is a genetic code for what?
A single gene can code for anything, we do not know. For example, if I picked 1 gene and then picked a second one, the first one could code for hair colour, where as the second one could code for freckles. In other words, a gene is a genetic code for a part of yourself.
Why is the E. coli lac Z gene used as a reporter gene in S. cerevisiae cells but not in E. coli cells?
The E. coli lac Z gene is used as a reporter gene in S. cerevisiae cells because the yeast lacks the lactose metabolism pathway, making it suitable for detecting lac Z expression. In contrast, E. coli naturally metabolizes lactose using the lac Z gene, so it is not ideal for use as a reporter gene in the same species.
How is the correct gene added to the cells?
The correct gene can be added to cells through a process called gene editing, where a specific gene is inserted into the cells using techniques like CRISPR-Cas9. This allows scientists to modify the genetic makeup of cells to introduce desired traits or correct genetic mutations.
What are the two broad areas of use and two examples after a host cell grown in culture to form a clone of cells containing the cloned gene of interest?
The two broad areas of use for cloned cells containing a gene of interest are medical research and biotechnology. Examples include using the cells to study gene function in disease models and to produce recombinant proteins for therapeutic purposes.
What cells don't contain 2 copies of each gene?
Gametes (sperm and egg cells) only contain one copy of each gene. This is because during meiosis, the cells divide to create haploid cells with half the number of chromosomes, resulting in genetic diversity when these cells combine during fertilization.
Why gene gun use in rice transformation?
It's a tool to insert foregin gene into target cells and can be used for any suitable crop or cells for transformation purpose. Now a days, agrobacterium mediated transformation techniques are becoming popular.
What is a homozygus?
When two cells have the same gene type then they are considered homozygous.
