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To avoid denaturing and destroying the smear.

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16y ago

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What would be the result of overheating the bacterial smear?

Overheating the bacterial smear can result in distortion or destruction of the bacterial cells, making it difficult to observe them under the microscope. This can lead to inaccurate or inconclusive results when trying to identify the bacteria present on the smear.


Why is it necessary to excessively rinse the smear with water in experiment identifying bacteria using gram stain?

smear should be rinsed with distill water so that all the small particles attach to the smear will washed away such as some time the crystals of dye are attached to the smear which give the illusion of microbial cell some time, distill water is used because it is free from other microbial cell and ions which can harm the smear.


What will happen when a thick smear is stained and viewed under a microscope?

When a thick smear is stained and viewed under a microscope, individual cells will be harder to distinguish due to overlapping. This can make it challenging to see details within individual cells, such as the internal structures or boundaries. It is typically recommended to use a thin smear for better visualization and interpretation of cellular details.


What is the purpose of covering the smear with bibulous paper when performing the endospore stain?

Covering the smear with bibulous paper during the endospore stain process helps to wick away excess stain and prevent the slide from drying out. This ensures that the endospores are properly stained and the background is clear for observation under the microscope.


What is a cervical smear?

A cervical smear, also known as a Pap smear, is a screening test used to detect abnormalities in the cells of the cervix that could potentially lead to cervical cancer. During the procedure, cells are collected from the cervix and examined under a microscope for any signs of pre-cancerous or cancerous changes. It is a routine test recommended for women to help prevent cervical cancer.