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The heat breaks the hydrogen bonds that hold the nitrogenous bases together in the centre of the DNA molecule. However, the covalent bonds between the phosphate and deoxyribose sugar are not affected.

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Is RNA a double-stranded molecule?

No, RNA is a single-stranded molecule.


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No, single stranded DNA molecules aren't considered antiparallel. Antiparallel refers to the orientation of the two strands in a double-stranded DNA molecule, where one runs 5' to 3' and the other runs 3' to 5'. Single-stranded DNA is usually unidirectional and doesn't have a complementary strand to be antiparallel to.


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If you have a small gene that you wish replicated by pcr you add radioactively labeled nucleotides to the pcr machine after 3 replication cycles how many double-stranded DNA molecules do you have?

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