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Why does filtered RPMI culture medium still contaminated after 4 days although the container is not opened?
Wiki User
∙ 11y ago
Check your aseptic technique, thoroughly ethanol the outside of the bottle, especially the cap and neck. Verify the receiving container is sterile or autoclaved. Do not reuse filters. Otherwise, using a 0.22nm filter should eliminate any contamination.
Wiki User
∙ 11y ago
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What is purity testing of microorganisms?
purity testing of microorganism is to check weahter the microbial culture contain one type of cells of species or it get contaminated or mixed with other type.the best way to check this is by sub culturing and staining.
If you strongly suspect a nutrient agar slant culture is contaminated what method would you use to ascertain whether you are right or not?
You have to make subculture from this slant and after incubation you can observe how many types of microorganisms are present in the nutrient agar slant. If you have one colony shape so you have a pure nutrient agar slant but if you have more than one type of colonies so the nutrient agar slant is contaminated.
How could you tell the bacteria were growing in the culture?
Bacteria growing on a petri dish can have more than one bacteria growing on it, but different characteristics can be seen like cilia, motility and color. To know more specifically what those other bacteria strains are, get a sample and grow it on agar by itself.
Could you distinguish between axenic culture and pure culture?
a pure culture is the one that originate from a single cell and has same specie of an organism while axenic culture can originate from more than one cell.
What is a cell culture and how can a cell culture be useful to biologists?
A cell culture is where cells are grown under controlled conditions.
What is the difference between a mixed culture and a contaminated culture?
A mixed culture is a container that holds two or more identified and easily differentiated species of microorganisms. A contaminated culture was once pure or mixed (and thus a known entity) but since had contaminants (unwanted microbes of uncertain identity) introduced into it.
Rave culture began?
The rave culture began in the 1980s in Europe and filtered to different parts of the world
What characteristics does a successful wound culture have?
A normal culture may be contaminated by a mixture of microorganisms normally found on a person's skin (normal flora).
How can you contract salmonellosis?
If a culture of salmonella is left open to the air it can become contaminated with other airborne bacteria.
A type of glass container commonly used for culture of bacteria?
Petri dish
Why must a sterile container be used if a urine culture id ordered?
Because an unsterile container may carry other elements that will confuse the outcome of the results.
Why should a petri dish not be left open for any extended period?
Other bacteria and germs could contaminate the contents in the dish. The dish is meant to isolate a subject in an experiment so one can study it. While in the dish, one can control the reactions by putting in what they want in the dish. If the dish is left open, then one will be studying the contaminates and will not get accurate results from their experiment.
What is pitridish?
A petri dish is a round, shallow container made of glass or plastic. It is used to culture bacteria and tissues.
Did the culture of Puerto Rico change over time?
In some way, yes, although the essence of their culture remains.
The although conquered by Romans would not allow Roman culture to have an influence on them.The although conquered by Romans would not allow Roman culture to have an influence on them.?
Greeks to a small extent. The Jews were another major group to resist Roman culture.
Why inoculum is take from edge not from center?
The bacteria on the margin are the most active and healthiest because they have access to fresh nutrients and have not yet contaminated the culture medium with waste.
What culture did Alexander the great admire?
Although a Macedonian, he was raised in Greek culture under the tutorship of Aristotle.
