0
depends what you intend to do, usually in a pcr reaction it denatures the DNA so you get 2 seperated strands
Wiki User
∙ 12y ago
Yostina Nasr
to seperate DNA into single strand to able to copy DNAthat occur in denaturation step of PCR reaction
Add your answer:
Earn +20 pts
What is the purpose of a PCR machine?
A PCR machine also known as a thermal cycler is a machine used to amplify segments of dna via the PCR which stands for polymerase chain reaction. PCR machines may also be used to test temperature sensitive reactions. The first step of the machine is to heat the samples to 94-96 degrees, then the temperature is lowered to 50-65 degrees, then the mixtures temperature is raised to 72 degrees to synthesize a new dna strand.
What temperature the animal DNA denatures?
Simply put, heat will denature DNA. Technically speaking, DNA has something known as the the melting temperature (which is the temperature at which a strand of DNA is separated halfway). And melting temperature is itself dependent on several other factors (like G to C ratio, salt conent, and pH).
What causes thymine dimers?
Excessive heat. When cells are exposed to sunlight, radiant energy can damage DNA.
A source of heat-stable DNA polymerase is?
A DNA polymerase is one of the crucial enzymes when DNA is synthesised. It is also the only enzyme needed when making DNA in the test tube, using a molecular biology technique known as PCR.In this reaction, the other enzymes that nature uses are are replaced by cycles of heating and cooling, up to 95 degrees Celsius. The DNA polymerase consists of protein, so normal DNA polymerase is of course destroyed in the heating step. The first PCR's were performed by opening the tube in each step and adding a tiny amount of fresh enzyme. Using heat-stable DNA polymerases made the technique a lot more practical. The enzymes used were taken från a type of archae (not exactly a bacterium, but almost) that live in hot springs and whose proteins all are very stable even in extreme temperatures. The archaeon is called "Thermus aquaticus", hence the name of the common lab DNA polymerases "Taq polymerase":To sum it up. A heat-stable DNA polymerase is a kind of DNA polymerase found in archaea living in hot springs, and of much use in the molecular biology lab.
What is the process of copying DNA to make two identical DNA molecules called?
polymerase chain reaction first scientists will put a primer at the beginning and end of each DNA strand. then they heat it to separate it's 2 strands then cool to bind single stranded DNA. then the DNA polymerase starts making copies of the region between the primers.
What is the steps in PCR?
1. Denaturation (separation of two strands of DNA by temperatures of around 94 to 98 degrees Celsius)2. Annealing (binding of DNA primer to the separated strands. Occurs at 50 to 65 degrees Celsius, which is lower than the optimal temperature of the DNA polymerases)3. Elongation (elongation of the strands using the DNA primer with heat-stable DNA polymerases, most frequently Taq (Thermus aquaticus) or Pfu (Pyrococcus furiosus) polymerases. Occurs at over 70 degrees Celsius)
How much is 94 degrees Celsius in Fahrenheit degrees?
94 degrees Fahrenheit is 34.44 degrees Celsius.
What is 94 degrees F in Celsius?
94 degrees Fahrenheit = 34.44 degrees Celsius
How many degrees centigrade is 94 farhenheheit?
94 degrees Fahrenheit is 34.44 degrees Celsius.
What is the purpose of a PCR machine?
A PCR machine also known as a thermal cycler is a machine used to amplify segments of dna via the PCR which stands for polymerase chain reaction. PCR machines may also be used to test temperature sensitive reactions. The first step of the machine is to heat the samples to 94-96 degrees, then the temperature is lowered to 50-65 degrees, then the mixtures temperature is raised to 72 degrees to synthesize a new dna strand.
My 94 explorer won't blow heat?
my 94 explorer wont blow heat
What is the angle of a triangle if the other two angles are 94 degrees and 56 degrees?
The 3rd angle is: 180-94-56 = 30 degrees
What angle is 94 degrees?
An angle of 94 degrees is an obtuse angle because it is greater than 90 but less than 180 degrees
What does heat do to the DNA polymerase?
Heat denatures protein. DNA polymerase is an enzyme and a protein.
What is 94 degrees Fahrenheit converted to celsius?
34.44 degrees Celsius.
What is the role of heat in DNA extraction?
Heat anneals DNA strand i.e. separate two strands of DNA to build anti-codon to desired DNA strand
What is 50 degrees north latitude and 94 degrees west longitude?
50 N 94 W is Favel Lake, Ontario, Canada.
