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depends what you intend to do, usually in a pcr reaction it denatures the DNA so you get 2 seperated strands

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What is the purpose of a PCR machine?

A PCR machine also known as a thermal cycler is a machine used to amplify segments of dna via the PCR which stands for polymerase chain reaction. PCR machines may also be used to test temperature sensitive reactions. The first step of the machine is to heat the samples to 94-96 degrees, then the temperature is lowered to 50-65 degrees, then the mixtures temperature is raised to 72 degrees to synthesize a new dna strand.


At what temperature do humans die from extreme heat or cold?

Humans can die from extreme heat at temperatures above 104 degrees Fahrenheit (40 degrees Celsius) and from extreme cold at temperatures below -40 degrees Fahrenheit (-40 degrees Celsius).


Can humans survive in temperatures of 150 degrees?

No, humans cannot survive in temperatures of 150 degrees Fahrenheit. Such extreme heat can lead to heat stroke, organ failure, and death.


What are the differences between heat shock and electroporation methods for transforming cells?

Heat shock and electroporation are two methods used to transform cells by introducing foreign DNA into them. Heat shock involves briefly exposing cells to high temperatures, which increases their permeability and allows the foreign DNA to enter. Electroporation, on the other hand, uses an electric field to create temporary pores in the cell membrane, through which the foreign DNA can pass. In summary, the main difference between heat shock and electroporation methods is the mechanism by which they make cells more receptive to foreign DNA.


How much heat can a human body withstand before experiencing harm or injury?

The human body can withstand temperatures up to 104 degrees Fahrenheit (40 degrees Celsius) before experiencing harm or injury. Beyond this point, heat-related illnesses such as heat exhaustion or heat stroke can occur.

Related Questions

What is the steps in PCR?

1. Denaturation (separation of two strands of DNA by temperatures of around 94 to 98 degrees Celsius)2. Annealing (binding of DNA primer to the separated strands. Occurs at 50 to 65 degrees Celsius, which is lower than the optimal temperature of the DNA polymerases)3. Elongation (elongation of the strands using the DNA primer with heat-stable DNA polymerases, most frequently Taq (Thermus aquaticus) or Pfu (Pyrococcus furiosus) polymerases. Occurs at over 70 degrees Celsius)


How much is 94 degrees Celsius in Fahrenheit degrees?

94 degrees Fahrenheit is 34.44 degrees Celsius.


What is 94 degrees F in Celsius?

94 degrees Fahrenheit = 34.44 degrees Celsius


How many degrees centigrade is 94 farhenheheit?

94 degrees Fahrenheit is 34.44 degrees Celsius.


My 94 explorer won't blow heat?

my 94 explorer wont blow heat


What is the angle of a triangle if the other two angles are 94 degrees and 56 degrees?

The 3rd angle is: 180-94-56 = 30 degrees


What is the purpose of a PCR machine?

A PCR machine also known as a thermal cycler is a machine used to amplify segments of dna via the PCR which stands for polymerase chain reaction. PCR machines may also be used to test temperature sensitive reactions. The first step of the machine is to heat the samples to 94-96 degrees, then the temperature is lowered to 50-65 degrees, then the mixtures temperature is raised to 72 degrees to synthesize a new dna strand.


What does -70 degrees Celsius equal in Fahrenheit?

-70 degrees Celsius is equal to -94 degrees Fahrenheit.


What angle is 94 degrees?

An angle of 94 degrees is an obtuse angle because it is greater than 90 but less than 180 degrees


What is -94 degrees Fahrenheit in Celsius?

Temperature is easy to convert from Fahrenheit to Celsius yourself. You can use the formula Tc = (5/9)*(Tf-32) where Tc = temperature in degrees Celsius, Tf = temperature in degrees Fahrenheit. -94 F is -137 C.


What is 94 degrees Fahrenheit converted to celsius?

34.44 degrees Celsius.


What is 50 degrees north latitude and 94 degrees west longitude?

50 N 94 W is Favel Lake, Ontario, Canada.