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TSA plates do not allow for the growth of anaerobes, becasue the entire culture is exposed to oxygen. So the TSA plates require the gaspak to provide an anaerobic condition.

FTM does allow for the growth of anaerobes and aerobes. Anaerobes will be found deep in the soln. away from the oxygen, and aerobes will be found at the top, with facultatives inbetween.

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Q: Why is a gaspak anaerobic jar necessary for the culture of anaerobes on TSA plates but not in tubes of FTM?
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